猪链球菌2型毒力因子与MLST分型分析

Analysis of Virulence Factors and Multilocus Sequence Typing Scheme of Streptococcus Suis Serotype2

为了解我国猪链球菌2型毒力因子和MLST型的特点,对四川、重庆和山东等地的22株猪链球菌2型进行毒力因子检测和ST型分析。结果显示,78%(17/22)的分离株为sly+mrp+epf+基因型的强毒株,仅山东省的5株分离株为弱毒力型。四川省和重庆市89%(15/17)的分离株为毒力较强的ST7型,而山东省的分离株均为毒力稍弱的ST1型。结合这些地区的猪链球菌病发病情况,分析提示猪链球菌2型的毒力因子和ST分型与菌株的致病性有一定的相关性。

To understand the characterization of virulence factors and multilocus sequence typing scheme of Streptococcus suis serotype 2,22 Streptococcus suis serotype 2 isolates from Sichuan,Chongqing and Shandong were detected in the virulence factors and multilocus sequence typing scheme analysis. The results showed that 78%（17/22）isolates were high virulent with sly＋ mrp＋ epf＋ genotype. Only 5 strains isolated from Shandong were low virulent. 89% isolates from Sichuan and Chongqing were high virulent ST7 type,but the isolates from Shandong were all lower virulent ST1 type. Comprehensive analysis of the research results and the occurrence situation of swine streptococcsis showed that the virulence factors and multilocus sequence typing scheme were related to the pathogenicity of Streptococcus suis serotype 2.