人线粒体转录终止因子2基因启动子区的生物信息学分析

BIOINFORMATICS ANALYSIS OF HUMAN MTERF2 GENE PROMOTOR REGION

人线粒体转录终止因子2基因(mitochondrial transcription termination factor 2,MTERF2)在线粒体基因表达、细胞能量代谢及细胞周期调控过程中发挥重要作用,但MTERF2基因自身的表达调控机制仍不明确。通过生物信息学方法预测和分析人线粒体转录终止因子2基因启动子区的结构与功能,结果显示:人MTERF2基因全长9876 bp,含有3个外显子和2个内含子。基因上游5’端2500 bp的核苷酸序列存在3个启动子,启动子区序列中存在长为874 bp的CpG岛,启动子区共发现244个转录因子结合位点。人MTERF2基因启动子区的生物信息学分析,提高了针对该基因启动子的研究效率,并为分析基因启动子的功能奠定了重要的理论基础。

Human mitochondrial transcription termination factor 2（MTERF2） plays an important role in mitochondrial gene expression, energy metablism and cell cycle regulation. However, the potential regulation mechanism of MTERF2 gene expression was still undiscovered. Online bioinformatics tools were used to analyze human MTERF2 gene＇s promotor. The results showed that the full length pf human MTERF2 gene was 9876 bp, which consist of three exons and two introns. There were three promoters in the 5＇ unconding region of human MTERF2 gene, two of which played a key role in the transcription of MTERF2. A 874 bp CpG island was found in human MTERF2 gene promoter region. In addition, total 244 transcription factor binding sites were found by Ali Baba 2.1 protocol. Gene promoter related bioinformatics can improve the efficiency of human MTERF2 gene promoter research, and provide significant information for the prediction of gene promoter function.