miRNA-21转录调控因子的生物信息学分析

Bioinformatics Analysis of Transcription Factors of mi RNA-21

【目的】探讨mi RNA-21转录调控因子AP-1、STAT3、NFI1、CBP、EBP、TGF-β、BMP-6、GFI1的结构特征及其与mi RNA-21转录结合位点。【方法】利用生物信息学工具分析mi RNA-21转录调控因子的理化性质、三级结构、跨膜区、信号肽、亚细胞定位及预测与mi RNA-21转录结合位点。【结果】等电点分析表明STAT3等电点小于7.0,其余7个转录调控因子等电点大于7.0;脂溶指数分析显示,EBP脂溶指数大于100,为亲水性蛋白,其余7个转录调控因子脂溶指数均小于100,为疏水性蛋白;从不稳定指数分析结果可知,EBP不稳定指数小于40,为稳定蛋白,其余7个转录调控因子不稳定指数大于40,为不稳定蛋白。所有转录调控因子二级结构均由α-螺旋、无规则卷曲和扩展链结构3种形式组成;EBP有跨膜区,BMP-6有信号肽,其余均无跨膜区或信号肽。亚细胞定位分析发现BMP-6位于细胞质中,EBP则位于质膜上,其余转录调控因子位于细胞核中;所有转录调控因子三级结构呈现发夹型结构,并预测出6个转录调控因子与mi RNA-21转录结合位点。【结论】mi RNA-21转录调控因子呈现多样性特征,拥有典型的发夹型结构特征,可能有助于对mi RNA-21转录表达调控,研究结果将有助于开展研究基于mi RNA-21的癌症治疗及其相关治疗药物的筛选。

【Objective】 To explore the structure characteristics and binding sites of transcription factors of mi RNA-21 including AP-1, STAT3, NFI1, CBP, EBP, TGF-β, BMP-6 and GFI1. 【Methods】 Physical and chemical characteristics, three-dimensional structure, subcellular localization, and binding sites were analyzed using the bioinformatics tools for transcription factors of mi RNA-21. 【Results】 Isoelectric point of most transcription factors are greater than 7.0. Aliphatic index analysis showed that EBP protein was hydrophilic and the others were hydrophobic. The instability indices revealed that most of transcription factors of mi RNA-21 were unstable. The secondary structures of corresponding all transcription factors were comprised of α-helix, random coil, and extended chain. Subcellular localization analysis implied that most transcription factors might exist in the nucleus. All transcription factor presents a hairpin structure in the level of tertiary structure, and six transcription factors were predicted for binding site. 【Conclusion】Tr anscription factors of mi RNA-21 have the typical characteristics of hairpin structure, which may contribute to regulate the transcription of mi RNA-21. Our results will contribute to the development of cancer treatment and drug design based on the mi RNA-21.