摘要
目的:探讨流动育龄妇女稽留流产的病因机制以及临床意义。方法:选取2013年5月-2015年5月来医院就诊的流动育龄妇女稽留流产患者共120例为观察组,并选择同期无自然流产史者行人工流产的流动育龄妇女正常孕妇100例为对照组。取2组的胚胎组织物标本,采用RT-PCR法检测胚胎组织中支原体(UU)、衣原体(CT)的基因拷贝数,并对胚胎组织采用FISH技术进行染色体变异分析。结果:观察组CT、UU以及CT+UU混合感染阳性率明显高于对照组,差异有非常显著性(P<0.01)。观察组胎儿绒毛组织的染色体正常72例,占60.0%;染色体数目异常48例,异常率为40.0%;对照组胎儿绒毛组织的染色体正常89例,占89.0%;染色体数目异常11例,异常率为11.0%。观察组胎儿绒毛组织的染色体异常率明显高于对照组(P<0.01)。结论:孕妇CT感染、UU感染以及两者的混合感染与稽留流产的发生密切相关,而胚胎染色体异常是稽留流产发生比较重要的因素。
Objective: To study the cause and clinical significance of childbearing age women with missed abortion. Methods: From May 2013 to May 2015,120 cases of childbearing age women with missed abortion were chosen as an observation group,and 100 cases of normal pregnant women,who had no history of spontaneous abortion and underwent induced abortion,were selected as a control group. The embryonic tissue specimens of the two groups were collected. The gene copy number of UU and CT in the embryonic organization was detected by RT-PCR in the two groups,and chromosome mutation analysis was carried out by FISH. Results: The infection positive rates of CT,UU and CT + UU mixed in the observation group were significantly higher than those in the control group( P〈0. 01). The number of normal fetal villi tissue chromosome in the observation group was 72( 60. 0%) and the abnormal chromosome number was 48( 40. 0%). Normal fetal villi tissue chromosome in the control group was 89( 89. 0%) and the abnormal chromosome number was 11( 11. 0%). The chromosome abnormality rate of fetal villi tissue in the observation group was obviously higher than that of the control group( P〈0. 01). Conclusion: The infection of CT,UU and CT + UU mixed of pregnant women is closely related with the occurrence of missed abortion. Embryo chromosome abnormality is one of the more important factors of missed abortion.
出处
《淮海医药》
CAS
2016年第2期142-143,146,共3页
Journal of Huaihai Medicine
基金
广东省东莞市科技计划医疗卫生类科研项目(2013105150000069)
关键词
稽留流产
病因
临床意义
流动育龄妇女
Missed abortion
Cause of disease
Clinical significance
Migrant women of childbearing age