样品保存温度和时间对总RNA提取的影响

Influence of total RNA extraction from samples stored in different temperature and time

目的:保障提取总RNA质量的前提下探索一种保存样品的适宜方法。方法:Trizol法(试剂盒)提取缺血复灌后肾组织总RNA。缺血45分钟后再灌注的大鼠肾脏,按照不同保存方法将样品分成四组,第一组液氮处理后立即提取总RNA;第二组-20℃冻存1天后提取总RNA;第三组-20℃冻存1周后提取总RNA;第四组液氮处理后1周后提取总RNA,然后检测各组RNA含量。结果:第一组RNA含量最多,第三组提取总RNA的含量最少。结论:提取总RNA的肾组织样品最佳保存方法是获得组织后立即提取总RNA,或者经液氮处理后保存在-20℃,一周后仍可获得较多含量的总RNA。

Purpose： looking for a good method to store the sample which can get the high quality total RNA. Methods： total RNA extraction（ Trizol kit） from rat ischemic kidney. The kidney cut from the rat after ischemia / reperfusion 45 min,samples store for four groups： 1. the RNA extracts by total RNA extraction kit immediately after the sample treated by liquid nitrogen; 2. the RNA extracts by total RNA extraction kit one day later and the sample store at-20℃; 3. the RNA extracts by total RNA extraction kit one week later and the sample store at-20℃; 4. the RNA extracts by total RNA extraction kit one week later and the sample store at liquid nitrogen. Result： Group one is the most and the group three is the least. Conclusion： extract the total RNA immediately is the best method,or week later after the sample store at-20℃ with liquid nitrogen treating,will still obtain more total RNA.