一种有效的图像处理方法对贮存红细胞闪烁复杂性的评估（英文）

Evaluatingthe flickeringcomplexity of the stored red blood cells usingan efficient imageprocessingmethod

体外红细胞老化研究具有极高的临床价值.临床应用上的不足,是由于缺少从微观帧上获取大量闪烁数据的有效方法.研究了一种新的图像处理方法,从不同的微观帧上提取红细胞灰度强度变化情况,使得细胞分裂能够在一张图像上显示.尺度不变特征转换被用来移除摄动的细胞.通过多尺度样本熵对来自25个年轻成年人(每人200个细胞)的红细胞膜闪烁的复杂性进行分析,同时,也测量三磷酸腺苷浓度.结果显示,提出的图像处理方法可靠且能够有效提取红细胞闪烁强度,红细胞老化过程能够通过膜闪烁的复杂性分析检测到,该过程比三磷酸腺苷测量要敏感得多.

Aging of the in-vitro red blood cell（RBC）is a topic with great clinical interests.The gap for its clinical application was the lack of the efficient and effective method to obtain the massive flickering data from the microscopic frames.A new image processing method was developed to extract the grayscale intensity variation of the RBCs from different frames.By the method,the cell segmentation could be performed on one image.Scale-invariant feature transformation was used to remove the perturbed cells.The flickering complexity of the RBCs from 25 young adults（200cells for each）was analyzed by multi-scale sample entropy.Meanwhile,adenosine triphosphate（ATP）concentration was measured.The results showed that the proposed image processing method was reliable and efficient to extract the flickering intensity of the RBCs.The aging process of the stored RBCs could be detected using the complexity analysis of the membrane flickering,which was much sensitive than ATP measurement.