重庆地区汉族人群FGF23基因多态性与冠心病的相关性分析

Correlation of FGF23 genetic polymorphism and coronary heart disease in Chongqing Han population

目的探索重庆地区汉族人群中成纤维细胞生长因子23（FGF23）基因多态性同冠心病发病的相关性。方法筛选2014年5月至2015年3月在我科接受冠脉造影检查的重庆汉族人群431例,其中冠心病（CHD）组231例与对照组200例。提取血液DNA样本后采用Sequenom Massarray系统对于rs7955866、rs13312756、rs3812822这3个FGF23基因Tag SNP位点进行基因分型,对其等位基因、基因型及单体型的组间分布进行统计学分析。结果 CHD组rs7955866 A等位基因和rs3812822 C等位基因频率明显高于对照组（P〈0.001）,两位点组间基因型分布也存在明显差异（P〈0.01）。单因素Logistic回归分析示rs7955866 GA基因型携带者患病风险为GG基因型的2.146倍,具有统计学意义（P=0.01,OR=2.146,95%CI[1.393~3.306]）,rs3812822 CT基因型与TT基因型携带者患病风险亦具有统计学差异（P=0.01,OR=2.010,95%CI[1.327~3.046]）。多因素非条件Logistic回归分析调整性别、年龄、BMI、吸烟饮酒史、高血压病史、高胆固醇血症等混杂因素后,结果显示rs7955866及rs3812822位点均与CHD发病独立相关（P〈0.001,OR=2.478,95%CI[1.613~3.806];P〈0.001,OR=2.123,95%CI[1.439~3.132]）。单体型rs7955866-rs13312756-rs3812822统计学分析结果显示,单体型ACC具有增加冠心病患病风险的作用（P〈0.001;OR=2.074,95%CI[1.391~3.091]）。结论重庆汉族人群FGF23基因多态性及与冠心病患病风险相关。

Objective To explore the correlation of fibroblast growth factor 23 （ FGF23 ） genetic polymorphism and coronary heart disease （CHD） in Chongqing Han population. Methods A total of 431 subjects were selected from the Department of Cardiology, from May 2014 to March 2015, and were divided into CHD group （ n = 231 ） and control group （ n = 200 ）. All the subjects were unrelated Han populations, and were examined by coronary angiography. Sequenom Massarray system was used for genotyping 3 Tag single-nucleotide polymorphisms （TagSNPs） of FGF23 gene, namely, rs7955866, rs13312756, and rs3812822. The frequencies of alleles, genotypes, and haplotypes of the TagSNPs were compared between the CHD group and the control group. Results The frequencies of rs7955866 A allele and rs3812822 C allele in the CHD group were significantly higher than those in the control group （P 〈0. 001 ）, and the genotype distribution of the 2 loci was significantly different between the 2 groups （ P 〈 0. 01 ）. Univariate logistic regression analysis showed that the risk of CHD in the subjects carrying rs7955866 GAgenotype was 2. 146 folds that of the ones carrying GG genotype （P =0. 01, OR =2. 146, 95% CI： 1. 393 3. 306）, and the subjects with rs3812822 CT genotype had higher CHD risk than the ones with Tr genotype （P = 0.01, OR = 2. 010, 95% CI： 1. 327 - 3. 046）. After adjusting the confounding factors like gender, age, BMI, smoking history, drinking history, history of hypertension and hypercholesterolemia, the results showed that SNPs at rs7955866 （P 〈 0. 001, OR = 2. 478, 95% CI： 1. 613 - 3. 806） and rs3812822 （P 〈 0. 001, OR = 2. 123, 95% CI： 1. 439 ~ 3. 132） were independently associated with CHD incidence. The ACC haplotype of rs7955866-rs13312756-rs3812822 had an effect of increasing CHD risk （P 〈 0. 001 ; OR = 2. 074, 95% CI： 1. 391 ~3. 091）. Conclusion FGF23 genetic polymorphism is related to the risk of CHD in Chongqing Han population.