免疫球蛋白重链结合蛋白和钙网蛋白在肝细胞癌中的表达与转移的关系

Relationship between the expression of immunoglobulin heavy chain binding protein and calreticulin and metastasis in hepatocellular carcinoma

目的探讨免疫球蛋白重链结合蛋白（Bip）和钙网蛋白（CRT）在肝细胞癌中的表达与转移的关系。方法采用Western印迹及实时荧光定量PCR技术检测Bip与CRT在正常肝脏细胞株及肝癌细胞株表达；收集2014年6月至2015年5月在郑州大学第一附属医院住院患者手术的肝细胞癌标本43例，用免疫组织化学染色和Western印迹技术检测43例肝细胞癌组织及相应的癌旁组织和5例肝血管瘤旁组织中Bip和CRT蛋白的表达，并分析与临床病理资料的关系。结果Bip和CRT在肝癌细胞株及肝细胞癌组织中高表达，在肝细胞癌组织中总的阳性表达率分别为86．0％（37／43）和65．1％（28／43），Bip在转移组高表达率为100％（19／19），明显高于非转移组的75．0％（18／24），两者比较，差异有统计学意义（P〈0．05）；CRT在转移组高表达率为84．2％（16／19），明显高于非转移组的50．0％（12／24），差异有统计学意义（P〈0．05）且两者表达呈正相关；Bip阳性率及表达量与肝细胞癌的TNM分期，转移相关（均P〈0．05），CRT阳性率及表达量均与肝细胞癌的转移相关（P〈0．05）。结论Bip和CRT的表达与肝细胞癌的转移相关，对肝细胞癌患者预后的监测有潜在意义。

Objective To investigate the relationship between the expression of immunoglobulin heavy chain binding proetin （Bip）and calreticulin （CRT） and metastasis in hepatocellular carcinoma. Methods Western blot and RT-qPCR were used to detect the expression of Bip and CRT in normal hepatic cells and hepatocellular carcinoma cells. 43 cases of hepatocellular carcinoma tissues were collected from the First Affiliated Hospital of Zhengzhou University between June 2014 and May 2015, Western blot and immunohistochemistry assay were used to detect the expression of Bip and CRT in hepatocellular carcinoma tissues, adjacent non-cancer tissues, 5 cases adjacent liver hemangioma tissues and the clinical significance was also analyzed. Results The expression of Bip and CRT in hepatocellular carcinoma ceils and hepatocellular carcinoma tissues was higher than normal hepatic cells and adjacent non- cancer tissues and also adjacent liver hemangioma tissues. The positive rate of Bip and CRT were 86. 0% （37/43） and 65.1% （ 28/43 ） in 43 hepatocellular carcinoma tissues, which was significantly higher than those adjacent non-cancer tissues and adjacent liver hemangioma tissues （P 〈 0. 05）. The positive rate of Bip and CRT in metastatic cancer tissues were 100% （19/19）,84.2% （16/19）, respectively, which was significantly higher than those non-metastatic cancer tissues 75.0% （ 18/24）, 50.0% （12/24） respectively （P 〈 0.05）. The expression of Bip and CRT was positively correlated（ r = 0. 382, P 〈 0. 05）. The positive rate and relative expression of Bip were correlated with TNM stage, metastasis in hepatocellular carcinomatissues（P 〈0. 05）, while the positive rate and relative expression of CRT were correlated with metastasis in hepatocellular carcinoma tissues （ P 〈 0.05 ）. Conclusions The expression of Bip and CRT is correlation with metastasis in hepatocellular carcinoma.