五劳七损方对强直性脊柱炎患者Dickkopf-1蛋白水平的影响

Effect of Wulao Qisun Formula on the Level of Dickkopf-1 of Ankylosing Spondylitis

目的:探索五劳七损方治疗强直性脊柱炎(AS)的有效性、安全性及可能的作用机理。方法:本研究应用ELISA的实验方法检测45例AS患者(AS组)治疗前后及50名健康人(健康组)血清Dickkopf-1(DKK-1)蛋白水平,并检测AS组治疗前后血沉(ESR)、C反应蛋白(CRP)含量及相关临床疗效评价指标。治疗过程中观察不良反应。结果:治疗前AS组血清DKK-1水平明显低于健康组(P<0.05),经五劳七损方治疗后血清DKK-1水平较治疗前明显升高(P<0.05),且与对照组比较差异无统计学意义(P<0.05)。治疗后ESR和CRP较治疗前均明显降低(P<0.05或P<0.01);相关临床疗效评价指标均较治疗前均明显改善(P<0.05)。患者治疗过程中未见明显不良反应。结论:五劳七损方改善AS患者临床症状、体征疗效显著,其作用机制可能是通过升高血清DKK-1水平实现的。

Objective：To observe the effect of Wulao Qisun formula on the level of Dickkopf-1（DKK1）in patients with ankylosing spondylitis（AS）.Methods：The level of DKK1 was detected before and after treatment in 45 cases of AS（AS group）and 50 healthy people（control group）by enzyme-linked immunosorbent assay method.The levels of erythrocyte sedimentation rate（ESR）,C-reactive protein（CRP）,and evaluation index of clinical efficacy were detected in AS group.The adverse reactions were observed in the treatment.Results：Before treatment,the level of DKK-1in the AS group was significantly lower than that of healthy group（P〈0.05）.After treatment,the level of DKK-1was significantly increased in the AS group（P〈0.05）and there was no significant difference between two groups（P〈0.05）.The levels of ESR and CRP,and the evaluation index of clinical efficacy were significantly improved after treatment（P〈0.05）.There was no obvious adverse reaction occurring during the treatment.Conclusion：Wulao Qisun formula is effective and safe for treating AS,and its mechanism may be achieved by increasing the level of serum DKK-1.