A型口蹄疫病毒(FMDV)VP1基因遗传进化分析

The Phylogenetic Analysis of Foot-and-mouth Disease Virus(FMDV)Serotype A Based on VP1 Gene

对疑似感染FMDV的样本进行了FMDV VP1基因RT-PCR鉴定,测定了其中1个阳性样本序列(命名为HY).采用生物信息学软件比较了HY与参考序列的同源性,并构建VP1基因的分子系统树.结果表明:HY属A型FMDV亚洲拓扑型东南亚-97谱系,与我国近年来报道的A/GDMM/CHA/2013的序列相似性为99.1%,与AF/72疫苗株的相似性仅为79.0%.提示我国近年来报道的A型毒株与AF/72疫苗株的序列差异较大,应及时更换A型口蹄疫疫苗毒株.

The samples suspected infection of FMDV were identified by RT-PCR method based on VP1 gene.One of the positive samples was sequenced and applied to homologous analysis with other reference sequences,to construct phylogenetic tree of VP1 gene.The results showed that the isolated VP1 belonged to serotype A,Asia topological type,and Sea-97 lineage.It has a high sequence similarity（99.1%）to A/GDMM/CHA/2013,a Chinese A type FMDV isolated in 2013,whereas has a low sequence similarity to the vaccine strain AF/72（79.0%）.The low sequence similarity between vaccine strain AF/72 and the A-type FMDV viruses reported recently indicated that AF/72 vaccine strain might only provide limited protection and the vaccine strain should be made up to date as soon as possible.