HPLC法测定黄芩免煎颗粒和饮片中黄芩苷的含量

Content Determination of Flavonoids in Scutellaria baicalensis Granule Preparation and Its Decoction Piece by HPLC

目的：比较黄芩免煎颗粒和饮片中有效成分黄芩苷的含量差异。方法：采用高效液相色谱法。色谱柱为Waters C18,流动相为甲醇-0.3%醋酸（47∶53,V/V）,流速为0.8 ml/min,检测波长为280 nm,柱温为25℃,进样量为20μl。结果：黄芩苷检测质量浓度线性范围为10-100μg/ml（r=0.999 9）;精密度、稳定性、重复性试验的RSD〈3%;加样回收率为95.48%-98.98%（RSD=1.26%,n=6,饮片）、95.76%-100.50%（RSD=1.75%,n=6,颗粒）。结论：该方法操作简便、稳定、重复性好,可用于黄芩免煎颗粒和饮片中黄芩苷含量的测定;各批次黄芩免煎颗粒与饮片中黄芩苷的含量无明显差异。

OBJECTIVE：To compare the different of contents of the active ingredient baicalin in Scutellaria baicalensis granule preparation and decoction piece. METHODS：HPLC was performed on the column of Waters C18 with mobile phase of methanol-0.3% acetic acid（47 ∶ 53,V/V）at a flow rate of 0.8 ml/min,the detection wavelength was 280 nm,the column temperature was 25 ℃,and the injection volume was 20 μl. RESULTS：The linear range of baicalin was 10-100 μg/ml（r=0.999 9）;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 95.48%-98.98%（RSD=1.26%,n=6,decoction piece） and 95.76%-100.50%（RSD=1.75%,n=6,granule）. CONCLUSIONS：The method is simple,stable and reproducible,and can be used for the content determination of baicalin in S. baicalensis granule preparation and decoction piece. There is no discernible differences between each kind of S. baicalensis granule preparation and decoction piece.