用纤维素键合型手性固定相分离6种β-受体阻断剂类药物对映体

Enantioseparation of six racemic β-blockers byhigh performance liquid chromatographyon immobilized cellulose chiral stationary phase

目的用HPLC手性固定相法分离6种β-受体阻断剂类药物对映体。方法使用Chiralpak IC和Chiralpak IB两种固定相,在正相色谱条件下对6种手性药物进行了分离研究。结果在Chiralpak IC柱上,贝凡洛尔、艾司洛尔、卡替洛尔、普萘洛尔能达到完全分离,美托洛尔达到部分分离,阿替洛尔不能分离;而在Chiralpak IB柱上,所有6种药物均能达到完全分离。结论 Chiralpak IB手性柱对6种β-受体阻断剂类药物表现了更好的对映体选择性。

Objective To establish a performance liquid chromatography method for the chiral separation of sixβ-blockers.Methods In the normal-phase mode,a chiral analysis of severalβ-blockers using HPLC with two cellulose-based chrial stationary phases,Chiralpak IC and Chiralpak IB,was established in this paper.Results Except Bevantolol,Esmolol,Carteolol and Propranolol were well separated from baseline,Metoprolol had partial separation,and Atenolol failed to be separated on Chiralpak IC.On the contrast,all of the six chiral drugs were successfully separated into enantiomers on Chiralpak IB.Conclusions In the case of separating theseβ-blockers,ChiralpakIB exhibit higher enantioseparation than Chiralpak IC.