HPLC法同时测定神康宁丸中4种成分的含量

Simultaneous determination of the content of four constituents in Shenkangning pills byHPLC

目的建立RP-HPLC法同时测定神康宁丸中斯皮诺素、迷迭香酸、紫草酸和丹酚酸B 4种成分的含量。方法采用高效液相色谱法（HPLC）,以Platisil ODS（250mm×4.6mm,5mm）为分析柱,乙腈-体积分数为0.1%的磷酸溶液为流动相进行梯度洗脱,流速：1.0mL·min-1,柱温：40℃,检测波长：340nm,进样量：20μL。结果斯皮诺素、迷迭香酸、紫草酸和丹酚酸B的质量浓度分别在1.032~10.32、0.460 0~4.600、1.736~17.36和21.12~211.2mg·L-1内与峰面积呈良好的线性关系（r≥0.999 6,n=6）。斯皮诺素、迷迭香酸、紫草酸和丹酚酸B平均回收率分别为100.9%、101.3%、100.4%和99.8%（RSD≤1.5%,n=9）。结论所建立的HPLC法可用于神康宁丸的质量控制。

Objective To develop an RP-HPLC wavelength switching method for simultaneous determination of spinosin,rosmarinic acid,lithospermic acid and salvianolic acid B in Shenkangning pills.Methods The HPLC separation was achieved on a Platisil ODS（4.6 mm×250 mm,5μm）column at 40 ℃.The mobile phase consisted of acetonirile-0.1% phosphoric acid solution with gradient elution at a flow rate of1.0mL·min-1.Results The linear range was 1.032-10.32 mg·L-1（r=0.999 9,n =6）,0.46-4.6 mg·L-1（r=0.999 8,n=6）,1.736-17.36 mg·L-1（r=0.999 6,n=6）,21.12-211.2 mg·L-1（r=1.000,n=6）,respectively.The recovery was 100.9%（RSD=0.7%,n=9）,101.3%（RSD=1.5%,n=9）,100.4%（RSD=1.0%,n=9）,99.8%（RSD=1.2%,n=9）respectively.Conclusions The method is simple,accurate and reproducible and can be used for quality control of Shenkangning pills.