摘要
目的本实验利用APP/PS1双转基因阿尔茨海默病(AIzheimer disease,AD)小鼠模型,观察神经节苷脂对AD小鼠学习记忆能力及海马磷脂酰肌醇3激酶(phosphoinositide 3-kinases,PI3K)表达的影响。方法 APP/PS1双转基因模型小鼠20只,随机分为AD模型组(10)和神经节苷脂(GM1)组(10),再取同窝阴性小鼠10只,作为对照组。经跳台试验和水迷宫试验进行行为学测试,用免疫组化方法和Western blot方法检测各组小鼠海马PI3K的表达变化。结果与对照组相比,AD模型组小鼠的学习和记忆成绩明显降低(P<0.05);相比于AD模型组小鼠,GM1组小鼠的学习和记忆成绩明显提高(P<0.05)。免疫组化和Western blot结果显示,GM1组小鼠和对照组小鼠海马PI3K蛋白阳性表达的平均光密度值显著高于AD模型组,P<0.05。结论 AD小鼠海马区PI3K的表达上调可能是GM1改善AD小鼠学习和记忆功能的机制之一。
Objective To study the effect of monosialoganglioside（GM1）on leaming and memory and the expression level of phosphoinositide 3-kinases（PI3K）in hippocampus of APP/PSl double transgenic mice of Alzheimer disease（AD）. Methods 20APP/PSl double transgenic mice were randomly divided into AD model group（10）,monosialoganglioside treatment group（10）and control group（10）. The behavioral scores were investigated by step-down test and water maze test.The expression of PI3K was examined in hippocampus of APP/PSl double transgenic by immunohistochemistry and western blot methods. Results Compared with AD group,the grades of learning and memory of mice in monosialoganglioside treatment group and control group were obviously increased（P〈0.05）;The mean optical density（MOD）of PI3K positive expression was higher of hippocampus in mice of monosialoganglioside treatment group and control groups than in mice of AD model group detected by immunohistochemistry method and western blot method. Conclusion The improvement of learning and memory abilities in AD mice might be related to the upregulation of PI3K expression in hippocampus caused by GM1.
出处
《解剖学研究》
CAS
2016年第1期28-31,共4页
Anatomy Research
基金
辽宁省科技攻关项目(No.2010225032)
