槲皮素对脑胶质瘤大鼠Akt和CREB表达的影响

Effect of quercetin on the expressions of Akt and CREB in rats with brain glioma

目的探讨槲皮素对脑胶质瘤大鼠Akt和CREB表达的影响。方法雌性Wistar大鼠30只,随机分为3组,即假手术组、模型组和槲皮素治疗组,每组10只。建立大鼠C6胶质瘤模型,于接种后17 d处死各组大鼠,采用免疫组织化学法和Western blot法检测肿瘤组织Akt和CREB蛋白表达水平的变化。结果与假手术组相比,模型组大鼠脑组织Akt和CREB的蛋白表达的平均光密度值显著升高,P<0.01;与模型组相比,槲皮素治疗组大鼠脑组织Akt和CREB的蛋白表达的平均光密度值显著降低,P<0.01。结论槲皮素能够降低脑胶质瘤大鼠Akt和CREB的表达。

Objective To study the effect of quercetin on the expressions of Protein kinase B（Akt）and c AMP response element binding protein（CREB）in rats with brain glioma. Methods A total of 30 female rats were randomly divided into 3 group（n =10 each group）：sham group,model group and quercetin treatment group. The rat model of C6 glioma was established,and animals were killed on 17 days after tumor graft.The expressions of Akt and CREB were observed by immunohistochemical method and Western blot methods. Results Compared with the sham group,The mean optic density（MOD）value of positive expressions of Akt and CREB in brain tissues of the rats in model group was higher significantly,P〈0.01;and compared with the model group,the MOD value of positive expressions of Akt and CREB in brain tissues of the rats in quercetin treatment group was lower significantly,P〈0.01.Conclusion quercetin could down-regulate the expressions of Akt and CREB in rats with brain glioma。