摘要
目的研究银杏内酯A对L-谷氨酸诱导HT22细胞损伤的影响。方法①体外培养HT22细胞,L-谷氨酸诱导细胞损伤,MTT法检测细胞存活率,确立L-谷氨酸对细胞损伤的浓度和时间依赖效应。②确立银杏内酯A对HT22细胞无毒范围,MTT法测定银杏内酯A对L-谷氨酸致HT22细胞存活率下降作用,蛋白质印迹检测银杏内酯A对凋亡相关因子表达和tau蛋白磷酸化水平的影响。结果①10 mmol/L L-谷氨酸与HT22细胞共孵育24 h是损伤模型的实验条件,银杏内酯A实验浓度范围内HT22细胞存活率无明显下降。②加入银杏内酯A共孵育后,细胞存活率出现明显上升,具有显性的差异,蛋白质印迹显示模型组Bax水平明显上升,Bcl-2水平明显下降,ptau(Ser202)水平明显上升,银杏内酯A干预组Bax水平明显下降,Bcl-2水平明显上升,ptau(Ser202)水平明显下降。结论高浓度的L-谷氨酸对HT22细胞有明显的损伤作用,且损伤呈时间和剂量依赖性,而银杏内酯A对损伤具有逆转作用,可以明显提高细胞的存活率。银杏内酯A对HT22细胞的保护作用可能与其降低Bax的表达,增加Bcl-2的表达,并降低磷酸化微管相关蛋白ptau(S202)的表达有关。
Objective This study aims to research the effects of ginkgolide A in L-glutamate induce HT22 cells injury.Methods ①To establish L-glutamate concentration and time dependence effect on cells injure,we cultivate HT22 cells in vitro,induced cells injury using L-glutamate and detect cell survival by MTT. ②We established ginkgolide A non-toxic scope to HT22 cells and detece the effect of ginkgolide A on HT22 cells survival rate by MTT. Western blot was performed to investigate the expression of apoptosis related factors and tau protein phosphorylation level. Results 1.10 mmol/L L-glutamate and HT22 cells were incubated with 24 h was the experimental condition of damage model and viability of HT22 cells didn＇t show significant differences in each concentration levels of ginkgolide A. ②Viality of ginkgolide A treatment group showed higher than model group. Western blot showed expression of Bax and ptau（ser202）in model group were higher while ginkgolide A treatment group appeared lower. On the other hand,expression of Bcl-2 was lower in model group than in ginkgolide A treatment group. Conclusion A high concentration of L-glutamate acid for HT22 cells have obvious damage with time and dose dependence. Ginkgolide A to injure has a reverse effect and significantly improve the cell survival rate. The mechanisms of ginkgolide A protection effect on HT22 cells may be in connection with up-regulation of Bcl-2 and down-regulations of Bax and ptau（s202）.
出处
《解剖学研究》
CAS
2016年第1期36-40,共5页
Anatomy Research
