96序列相似的家庭成员B基因在结肠癌组织中的表达及其临床意义

Down - regulation of family with sequence similarity 96, member B in colon cancer and its clinical significance

目的观察96序列相似的家庭成员B（FAM96B）在86例结肠癌组织中的表达，并探讨其临床意义。方法收集86例结肠癌患者的组织标本。分别采用实时定量聚合酶链反应（Real—timePCR）、Westernblot及免疫组织化学技术检测86例结肠癌组织及相应的癌旁组织中FAM96BmRNA及蛋白表达分布。并分析其表达差异与临床病理特征之间的关系。结果在86例结肠癌组织中，FAM96BmRNA在肿瘤组织中的表达显著低于癌旁组织（1．54±0．20比0．63±0．10，P〈0．05）。FAM96B蛋白在肿瘤组织中的表达同样低于癌旁组织（P〈0．05）。且在肿瘤组织中低表达的FAM96B与淋巴结转移、远处转移及TNM分期显著相关（P〈0．05），然而与性别、年龄、肿瘤大小、分化程度及病理类型无相关（P〉0．05）。免疫组织化学检测结果显示FAM96B在结肠癌组织中的蛋白表达定位于细胞质。且FAM96B在结肠癌与癌旁组织中的阳性表达率分别为77．9％（67／86）和29．1％（25／86），两者差异有统计学意义（P〈0．05）。结论FAM96B在结肠癌组织中低表达，其表达水平与淋巴结转移、远处转移及TNM分期有关。

Objective To investigate the expression difference of family with sequence similarity 96, member B （FAM96B） in 86 cases of colon cancer tissues and explore the clinical significance. Methods Eighty - six cases of colon cancer tissues and their adjacent tissues were collected from January 2014 to March 2015 in our hospital. Real - time quantitative polymerase chain reaction （ Real - time PCR） , Western blotting and immunohistoehemistry were used to examine the expression and distribution of FAM96B in colon cancer tissues and their adjacent tissues from 86 patients with colon cancer. Further more, the correlation between the expression difference of FAM96B and clinicopathological characteristics of colon cancer was analyzed. Results In 86 patients with colon cancer, FAM96B mRNA levels were sig- nificantly higher in tumor tissues than in adjacent tissues （ 1.54 ±0. 20 vs. 0. 63 ±0. 10,P 〈 0. 05 ）. The FAM96B protein expression levels in tumor tissues were lower than in adjacent tissues （ P 〈 0. 05 ）. The expression difference was correlated with lymph nodes metastasis, distant metastasis and TNM stage （P 〈 0. 05）, but not with gender, age, tumor size, differentiation or histologic subtype （P 〉 0. 05 ）. Immuno- histochemistry showed that FAM96B protein was located in cytoplasm. In addition, the positive expression rate of FAM96B in tumor tissues and adjacent tissues was 77. 9% （67/86） and 29. 1% （25/86） respec- tively （ P 〈 0. 05 ）. Conclusion Our data demonstrate that the expression of FAM96B in colon cancer tis- sues is significantly decreased as compared with that in adjacent tissues. The expression difference was cor- related with lymph nodes metastasis, distant metastasis and TNM stage. Additionally, FAM96B may be a good marker to evaluate the invasion and metastasis.