不同手术切除方案对转移性结肠癌小鼠预后的影响

Effects of different surgical procedures on prognoses of metastatic colorectal cancer mice

目的观察转移性结肠癌灶在不同手术切除方案实施后对小鼠预后的影响。方法采用CT26细胞建立皮下结肠癌移植瘤鼠模型，按切除转移结肠癌的方案不同分为非手术组、80％手术切除组、30％手术切除组，分别处理实验鼠。行为学监测实验鼠质量、脏器重量和肿瘤体积变化，形态学检测不同切除比例后肿瘤组织的上皮钙黏蛋白（E-cadherin，E—cad）及增殖细胞核抗原（Ki-67）等表达变化，酶联免疫吸附法测定血管内皮生长因子（VEGF）的含量。结果实验鼠肿瘤在切除80％之后小鼠质量变化率较切除30％稍低；80％切除组的脾脏指数[（3．1990±0．4059）％]较30％切除组[（5．7340±0．7320）％]明显下降（P〈0．05）；80％切除组肿瘤体积变化率[（279．700±27．370）％]较30％切除组[（40．390±7．663）％]明显增大（P〈0．05），非手术组的肿瘤体积变化率最小；80％切除组最终肿瘤组织瘤重[（0．37190±0．08551）g]较30％切除组[（1．59300±0．18160）g]明显减低（P〈0．05）；VEGF在不同手术切除组间差异无统计学意义（P〉0．05）；肿瘤免疫组织化学染色表明，E—cad含量在80％切除组；累计吸光度（M）值为（115200±9161）较30％切除组（49850±16200）明显升高（P〈0．05），而Ki-67在不同切除比例下差异无统计学意义（P〉0．05）。结论实验鼠在肿瘤切除80％之后预后较30％切除组好，提示肿瘤晚期的小鼠不能得到R0切除时，手术切除大部分肿瘤有助于改善预后。

Objective To observe the effect of different surgical procedures on prognoses of meta- static colorectal cancer mice. Methods CT26 cells were used to establish the metastatic colorectal cancer model in mice by subcutaneous xenograf. Then the mice were divided into three groups in terms of the sur- gical procedures： non- surgery group, 80% resection group, amd 30% resection group. Through the be- havioral surveillance, body weight, organ weight and tumor volume changes were recorded in the model mice. The expression of E - cadherin （ E - cad） and proliferation cell nuclear antigen （ Ki - 67 ） was as- sessed by the morphological examination. Enzyme linked immunosorbent assay （ELISA） was used to detect the content of vascular endothelial growth factor （VEGF）. Results The rate of change of mass in mice of 80% resection group was lower than in 30% resection group. The spleen index in 80% resection group [ （3. 199 0 ±0. 405 9）% ] was lower than in 30% resection group [ （5. 734 0 ±0. 732 0）% ]. The tumor volume change in 80% resection group [ （279. 700 ± 27. 370） % ] was larger than 30% resection group [ （40. 390 ± 7. 663） % ] （P 〈 0. 05）, and least in the non - surgery group. However, the final tumor tis- sue excised tumor weight in 80% resection group [ （0. 371 90 ± 0. 085 51） g] was significantly lighter than in 30% resection group [ （ 1. 593 00 ±0. 181 60） g] （P 〈0. 05）. VEGF levels showed no significant difference among the three groups （ P 〉 0. 05 ）. The immunohistochemistry showed that the IA of E - cad- herin protein in 80% resection group was 115 200 ± 9 161, significantly higher than that in 30% resection group （49 850 ± 16 200）. Ki- 67 showed no significant difference among the three groups （P 〉 0. 05 ）. Conclusion The metastatic colorectal cancer in mice has a better prognosis in 80% resection group than in 30% resection group, suggesting that advanced tumors in mice which can not get a R0 resection will also has a good advantage when most of the tumor is resected.