氧甾酮133和重组人骨形态发生蛋白-2对兔后外侧腰椎模型中腰椎融合率的比较

Effect of oxysterol 133 vs. recombinant human bone morphogenetic protein -2 on fusion rate in a rabbit posterolateral lumbar spine model

目的探讨氧甾酮133（Oxy133）和重组人骨形态发生蛋白-2（rhBMP-2）对兔后外侧腰椎模型融合率的影响。方法对24个月龄雄性成年新西兰白兔（3．0～3．5kg）的L3-L4脊椎实行双侧后外侧脊椎融合术。实验兔分成4组：对照组（A组）、30txgrhBMP-2（B组）、20mgOxy133（C组）、60mgOxy133（D组）。4周后，荧光显影检查其融合，8周后处死兔，通过放射显影、临床触诊和计算机断层扫描技术评估其融合效率。结果8周时放射显影技术进行融合率分析各组分别为：A组41．7％；B组91．7％；C组91．7％；D组100．O％。处死兔后剥离其脊柱通过临床触诊分析其融合率结果：A组0．0％；B组83．3％；C组83．3％；D组66．7％。计算机断层扫描结果进一步确定以上结果。结论20mg和60mgOxy133都能促进骨融合，融合效率基本与30斗grhBMP-2接近，均显著高于对照组，表明Oxy133有希望替代rhBMP-2成为促进骨融合的治疗剂。

Objective To compare the effects of recombinant human bone morphogenetic protein- 2 （rhBMP- 2） vs. oxysterol （Oxy）133 on fusion rate in a randomized controlled trial using a posterolateral lumbar rabbit spinal fusion model. Methods Twenty - four male adult white New Zealand rabbits （3.0 - 3.5 kg） underwent bilateral posterolateral lumbar spinal fusion at L4 - LS. Rabbits were di- vided into four groups： control （A）, 30 -mg rhBMP -2 （B）, 20 - mg Oxy 133 （C）, and 60 - mg Oxy 133 （D）. At 4st week, fusion was evaluated by fluoroscopy, and at 8th week, the rabbits were sacri- riced and fusion was evaluated radiographically, by manual palpation, and with microcomputed tomo- graphy. Results Radiographic analysis showed the rusion rate at 8th week in groups A, B, C and D was 41.7%, 91.7% , 91.7%, and 100.0% respectively. Evaluation of fusion masses by manual palpation of excised spines after sacrifice showed the fusion rate in group A, B, C and D was 0. 0%, 83.3% , 83.3% and 66.7% respectively. Microcomputed tomography scanning confirmed these findings. Conclusion These findings in a rabbit model demonstrate that both 20 - and 60 - mg Oxy 133 doses promote fusion that is equivalent to fusion induced by 30 - mg rhBMP - 2 and significantly greater than the control group. The present findings confirm that Oxy 133 is a promising candidate for therapeutic development as an alternative to rhBMP- 2 to promote spinal fusion.