低温对牡丹切花花色和花青素苷合成的影响

Effect of Low Temperature on Flower Color and Anthocyanin Biosynthesis in Tree Peony(Paeonia suffruticosa)‘Luoyang Hong'Cut Flower

以牡丹洛阳红(Paeonia suffruticosa‘Luoyang Hong’)开放级别为蕾开期的切花为材料,研究不同温度(22℃、15℃和4℃)处理对切花花色和花青素苷合成的影响。结果表明:与22℃处理相比,15℃和4℃处理切花花色明度下降、红度和彩度增加,花瓣花青素苷含量增加。对花青素苷合成相关基因表达量分析的结果表明:15℃和4℃低温促进与花青素苷合成相关的PsCHS1、PsCHI1、PsF3'H1、PsANS1、PsDFR1、PsMYB2、Psb HLH1和Psb HLH3基因的表达。低温对花青素苷上游合成途径中PsCHS1和PsCHI1基因进行调控;下游合成途径中PsDFR1、PsANS1和PsF3'H1基因对低温的响应相对敏感,4℃处理后基因的表达量大幅上调,且显著高于15℃处理组。上述所提到的结构基因和调节基因均是受低温调控的关键基因,进而影响牡丹洛阳红切花花青素苷合成与积累。

The cut flowers of Paeonia suffruticosa‘Luoyang Hong＇were used as materials. Effects of different temperature treatments（ 22 ℃,15 ℃ and 4 ℃） on flower color and anthocyanin biosynthesis were researched. The results showed that ： compeared with 22 ℃,flowers under 15 ℃ and 4 ℃ treatments showed lower lightness,higher redness,higher chroma and increased anthocyanin accumulation. Color quality was obviously better than that of22 ℃ treatment. qRT-PCR was used to analyze the relative expression levels of anthocyanin biosynthetic genes and results showed that 15 ℃ and 4 ℃ treatments promoted anthocyanin biosynthesis in the cut flowers via the promotion of related genes transcription（ PsCHS1,PsCHI1,PsF3＇ H1,PsANS1,PsDFR1,PsMYB2,Psb HLH1 and Psb HLH3）. Low-temperature mainly regulated and controled anthocyanin biosynthesis by upstream gene PsCHS1 and PsCHI1. Downstream genes PsDFR1,PsANS1 and PsF3＇H1 were sensitive to low-temperature,which hade the biggest increase transcription by 4 ℃ treatment. The above mentioned structural and regulatory genes were the important key of the anthocyanin biosynthesis.