辛伐他汀抑制机械牵张力介导的巨噬细胞活性氧族元素的表达

Simvastatin Repressed ROS Expression of Macrophage Cells Induced by Stretch Stress

目的探讨机械牵张力刺激能否介导小鼠巨噬细胞活性氧族元素(ROS)的产生,并进一步探讨辛伐他汀对此作用的影响及其作用机制。方法体外常规培养小鼠巨噬细胞RAW264.7,施加机械力刺激,或用辛伐他汀预处理细胞60 min后再施加此刺激,用荧光探针H2DCFDA检测巨噬细胞ROS的表达水平,以细胞免疫荧光定量阳性率和SPSS统计软件对其表达水平进行分析。Western blot检测NADPH氧化酶1(NOX1)的表达。结果机械牵张力刺激介导的巨噬细胞ROS呈时间依赖性增多,以60 min最显著(P<0.05);辛伐他汀可抑制此刺激介导的巨噬细胞ROS表达增加,且抑制作用呈浓度依赖增强,以0.3μmol/L辛伐他汀的抑制作用最显著(P<0.05)。辛伐他汀可使机械牵张力介导的巨噬细胞NOX1表达显著减少。结论辛伐他汀可通过抑制NOX1的表达从而抑制由机械牵张力介导的小鼠巨噬细胞ROS的表达。

Aim To investigate the effect of simvastatin on reactive oxygen species（ ROS） expression in stretch stress（ SS） induced macrophage cells. Methods RAW264. 7 macrophage cells were cultured in vitro,treated with SS,or pretreated with simvastatin for 60 min and then stimulated with SS,the expression of ROS was detected by Hoechst33342 and H2 DCFDA fluorescent probe,the fluorescence intensity of ROS was detected and the positive ratio of ROS was analysed by SPSS statistical software. The expression of NADPH oxidase 1（ NOX1） was evaluated by Western blot.Results SS increased the expression of ROS in a time dependent manner,and the expression of ROS reached the most significant at 60 min（ P〈0. 05）. Simvastatin could inhibit the effect induced by SS in a concentration dependent manner,the inhibition effect of simvastatin was the most significant in 0. 3 μmol / L（ P〈0. 05）. The expression of NOX1 was significantly inhibited by simvastatin after SS. Conclusion Simvastatin repressed ROS expression of RAW264. 7 macrophage cells induced by SS through inhibiting the expression of NOX1.