UPLC法测定冠脉康片中3种成分含量

Determination of Ginsenoside Rg_1,Rb_1 and Notoginsenoside R_1 in Guanmaikang Tablet by UPLC

目的：建立UPLC法同时测定冠脉康片中3种成分（三七皂苷R_1、人参皂苷Rg_1及人参皂苷Rb1）的含量。方法：采用Waters ACQUITY UPLCBEH C_（18）柱（5mm×210mm,1.7μm）,以乙腈-水溶液为流动相,梯度洗脱,流速为0.6mL·min-1,检测波长为203nm,柱温为25℃。结果：人参皂苷Rg1、人参皂苷Rb_1及三七皂苷R_1的线性范围分别为0.003 8~0.041 8μg（r=0.999 7）、0.062 4~0.686 4μg（r=0.999 7）、0.005 8~0.063 8μg（r=0.999 9）;平均加样回收率分别为101.24%、98.12%、100.33%;RSD（n=6）分别为1.34%、1.82%、1.55%。结论：该方法稳定、快速、专属性强,可用于冠脉康片中人参皂苷Rg1、人参皂苷Rb1及三七皂苷R_1的含量测定及其质量控制。

Objective：To establish a RP-UPLC method for simultaneous determination of Ginsenoside Rg1,Rb1 and Notoginsenoside R1 in Guanmaikang Tablet.Methods：The separation was performed on a Waters ACQUITY UPLCR BEH C18column（5mm×210mm,1.7μm）with gradient elution（0~4min,A：18%;4~5.5min,A：18%~20%;5.5~6.5min,A：20%;6.5~7min,A：20%~28%;7~12.5min,A：28%~30%;12.5~13min,A：30%~90%;13~16min,A：90 %）using acetonitrile（A）and water（B）as mobile phase at a flow rate of 0.6mL·min-1.The detection wavelengths was 203 nm.Results：Linear ranges of Ginsenoside Rg1,Rb1 and Notoginsenoside R1 were 0.003 8~0.041 8μg（r=0.999 7）,0.062 4~0.686 4μg（r=0.999 7）,0.005 8~0.063 8μg（r=0.999 9）,respectively.The average recoveries（n=6）of the three lignans were 101.24%,98.12%,100.33%;RSDs were 1.34%,1.82%,1.55%,respectively.Conclusion：The method is sensitive,accurate and can be used to determine the contents of Ginsenoside Rg1,Rb1 and Notoginsenoside R1 in Guanmaikang Tablet,which may also be applied as a standard for the quality control of in Guanmaikang Tablet.