剑麻皂素对2型糖尿病(T2DM)大鼠的治疗作用及其机制探讨

The Effects and Mechanism of Tigogenine on Streptozotocin-induced Diabetic Rats

目的:探讨剑麻皂素对T2DM大鼠的治疗作用及其作用机制。方法:高糖高脂饮食加小剂量链脲佐菌素(STZ)联合诱导制备T2DM大鼠模型。研究剑麻皂素对T2DM大鼠血脂、血糖、胰岛素、氧化应激反应、血管内皮功能、主动脉功能的影响。结果用药治疗12周后,剑麻皂素能明显降低糖尿病大鼠血清血糖(GLU)、总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、游离脂肪酸(FFA)、丙二醛(MDA)、血管性血友病因子(v WF)、糖基化终末产物(AGEs)含量;提高高密度脂蛋白(HDL-C)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPx)、一氧化氮(NO)、胰岛素(INS)含量;同时能改善糖尿病大鼠离体主动脉的收缩和舒张反应。结论:剑麻皂素具有调节T2DM大鼠血糖、胰岛素、血脂、主动脉功能等作用,其作用机制可能与改善机体的氧化应激和血管内皮功能有关。

Objective： To investigate the effects and mechanism of Tigogenine on streptozotocin-induced diabetic rats. Methods： High glucose and high fat diet, combined with small dose streptozotocin was used to establish type 2 diabetes rat mode. The changes of blood lipids, blood glucose, insulin release, oxidative stress,vascular endothelium and thoracic aorta in rats were observed. Results： After Tigogenine treatment for 12 weeks,the contents of blood GLU, TC, TG, LDL-C, FFA, MDA, v WF, AGEs in rats obviously decreased, and the levels of HDL-C, SOD, GSH-Px, NO, INS significantly increased. Tigogenine also improved the vasodilation and vasoconstriction function. Conclusion： Tigogenine has positive effects on the blood glucose, insulin, blood lipids and aortic function in T2 DM rats, which may due to its anti-oxidative and endothelial protective effects.