戊型肝炎病毒感染长爪沙鼠体内IL-10表达的研究

Expression of IL-10 in gerbils experimentally infected by hepatitis E virus

为了解IL-10在戊型肝炎病毒(HEV)感染过程中的作用,采用免疫组织化学方法对长爪沙鼠人工感染HEV,对攻毒后第3、5、7、14、21、28和35天的心、肝、脾、肺、肾、肠等组织中IL-10的表达水平进行了检测。结果发现,各组织中IL-10表达量在感染后第5天最大,以肺最高、肾次之;随后逐渐降低,第21天与对照组水平接近。HEV阳性物质主要分布于肝小叶内的肝细胞胞核及胞质内,有淋巴细胞浸润的肝组织周围分布更多。小肠上皮、肺泡上皮和心肌细胞胞质以及脾红髓、白髓等也较为常见。本研究为揭示戊型肝炎病毒的致病机理,抗病毒生物制剂研发等奠定了基础。

To determine the role of interleukin 10（IL-10） in pathogenesis of hepatitis E virus（HEV）infection,the expression levels of IL-10 at 3 days post inoculation（DPI）,5DPI,7DPI,14 DPI,21DPI and 28 DPI and 35 DPI in Mongolian gerbils experimentally infected with HEV were determined by immunohistochemical stain.The results showed that the expression of IL-10 in gerbils′ heart,liver,spleen,lung,kidney and intestine tissues were reach the highest in 5DPI,followed by the lung and kidney.At 21 DPI,the expression of IL-10 in all tissues have decline to baseline,compared to control group.The positive substance was usually distributed in both cytoplasm and nucleus of hepatocytes in the liver,especially around the lymphocyte infiltration tissues,in cytoplasm of small intestinal epithelium,alveolar epithelium and cardiomyocytes,also red pulp of spleen and white pulp of spleen.The results laid the foundation for revealing the pathogenesis of HEV infection,and research and development of anti-viral biological agents.