幽门螺杆菌感染与胃癌发生发展相关性研究

Correlation between Helicobacter pylori infection and occurrence and development of gastric cancer

目的探讨幽门螺杆菌(Hp)感染与胃癌发生发展的相关性,为临床预防与阻止胃癌的发生提供可靠的参考依据。方法选取2014年5月-2015年5月90例胃癌患者的胃癌组织标本作为研究组;并选其中40例胃癌患者旁癌组织标本作为对照组,且经过病理学诊断均为正常组织;采取荧光定量PCR相对定量法进行检测,研究两组患者Hp相对感染量,分析Hp与胃癌发生发展的关系,采取SPSS19.0软件进行统计分析。结果患者Hp相对感染量研究组为(9.5±2.1),对照组为(4.5±1.2),研究组Hp相对感染量显著的高于对照组,两组比较差异有统计学意义(P<0.05);研究组患者Hp相对感染量与不同临床TNM分期、不同淋巴结转移及浸润深度间比较,差异均统计学意义(P<0.05);两组患者性别、年龄、临床TNM分期、淋巴结转移、浸润深度及胃癌发生部位Hp感染率比较差异均无统计学意义。结论幽门螺杆菌感染与胃癌的发生发展有着密切的联系,且参与了胃癌的浸润深度和转移以及临床分期,并且影响胃癌的发生发展,临床中可以将幽门螺杆菌感染作为评估胃癌进展的重要标志物。

OBJECTIVE To explore the relationship between Helicobacter pylori infection and the occurrence and development of gastric cancer so as to provide guidance for clinical prevention of gastric cancer.METHODS The gastric cancer tissue specimens were collected from 90 patients with gastric cancer who were treated in the hospital from May 2014 to May 2015 and were chosen as the study group,and the paracancerous tissue specimens were obtained from 40 patients with gastric cancer and were set as the control group,all of which were normal tissues through the pathological diagnosis.The specimens were detected by using relative quantitative real-time PCR method,H.pylori relative infection amount of the two groups of patients was observed,the relationship between H.pylori infection and the occurrence and development of gastric cancer was analyzed,and the statistical analysis was performed with the use of SPSS19.0software.RESULTS H.pylori relative infection amount of the study group was（9.5±2.1）,significantly higher than（4.5±1.2）of the control group（P0.05）.There was significant difference in the H.pylori relative infection amount among the patients with different clinical TNM stages,lymph node metastasis,or depths of invasion in the study group（P0.05）.There was no significant difference in the incidence rate of H.pylori infection in the patients with different genders,age,clinical TNM stages,lymph node metastasis,invasion depths,or sites of gastric cancer between the two groups.CONCLUSION H.pylori infection is closely associated with the occurrence and development of gastric cancer,it may involve in the invasion depth and metastasis and clinical stage of the gastric cancer,and affect the development of gastric cancer.H.pylori infection can be served as an important clinical marker for evaluation of development of gastric cancer.