替米沙坦对病毒性心肌炎小鼠的心肌保护作用研究

Effect of telmisartan on protection of myocardia of mice with viral myocarditis

目的研究替米沙坦对病毒性心肌炎小鼠的心肌保护作用及机制,以减轻其心肌损伤,避免向扩张性心肌病发展。方法选取实验小鼠70只,随机分为观察组、对照组各30只及空白组10只;观察组和对照组腹腔注射柯萨奇B3病毒以制备心肌炎小鼠模型,观察组小鼠自病毒感染之日连续7d喂食替米沙坦,对照组无药物干预,空白组小鼠于腹腔注射等量磷酸盐缓冲液,同样无药物干预;比较3组小鼠的心肌病理组织学积分,血清脂联素、肌钙蛋白Ⅰ、BNP的浓度以及心肌匀浆液中白介素-6,肿瘤坏死因子-α的浓度。结果观察组血清脂联素水平明显高于对照组,心肌匀浆中白介素-6,肿瘤坏死因子-α,血清肌钙蛋白Ⅰ、BNP水平以及病理积分均低于对照组,差异有统计学意义(P<0.05);与空白组相比,对照组的血清脂联素降低而白介素-6、肿瘤坏死因子-α、肌钙蛋白Ⅰ、BNP以及病理积分水平明显升高,差异有统计学意义(P<0.05)。结论在小鼠模型中,替米沙坦可减轻病毒性心肌炎导致的心肌损伤,从而起到保护心脏的作用,其机制可能与血清脂联素水平升高,白介素-6、肿瘤坏死因子-α等炎症因子水平降低有关。

OBJECTIVE To study the effect of telmisartan on protection of myocardia of mice with viral myocarditis and its mechanism so as to alleviate the myocardial injury and avoid the development of dilated cardiomyopathy.METHODS A total of 70 mice were recruited as the experiment objects and randomly divided into the observation group with 30 mice,the control group with 30 mice,and the blank group with 10 mice.The observation group and the control group were treated with abdominal injection of coxsackie B3 virus so as to establish the myocarditis mice model,the mice in the observation group were fed with telmisartan from the date when they had the viral infection,for 7consecutive days;the control group was not given any drug intervention;the mice in the blank group were treated with abdominal injection of equal amount of phosphate buffer but were not given drug intervention.The myocardial pathologic histology integral,concentrations of serum adiponectin,cardiac troponin I（cTn I）,and BNP as well as concentrations of tumor necrosis factor alpha and interleukin-6in myocardial homogenates were observed and compared among the three groups.RESULTS The level of serum adiponectin of the observation group was significantly higher than that of the control group,however,the levels of tumor necrosis factor alpha and interleukin-6in myocardial homogenates,the levels of serum cardiac troponin Ⅰ and BNP,and pathologic integral were lower in the observation group than in the control group,and there was significant difference（P0.05）;as compared with the blank group,the level of serum adiponectin of the control group was reduced,while the levels of interleukin-6,tumor necrosis factor alpha,troponinⅠ and BNP,and pathologic integral were significantly elevated,and there was significant difference（P0.05）.CONCLUSIONTelmisartan can alleviate myocardial injuryinduced by the viral myocarditis and has certain effect on protection of hearts,and its mechanism may be associated with the elevated level of serum adiponectin,or the reduced levels of inflammatory factors such as interleukin-6and tumor necrosis factor alpha.