分泌性白细胞蛋白酶抑制剂在胃溃疡愈合中的作用机制探讨

Change of Connective Tissue Growth Factor in Gastric Mucosa during Healing of Gastric Ulcers

【目的】探讨分泌性白细胞蛋白酶抑制剂（secretory leucocyte protease inhibitor，SLPI）在胃溃疡愈合中的作用机制。【方法】采用免疫组织化学染色法、反转录聚合酶链反应（Reverse TranscriptionPolymerase Chain Reaction ，RTPCR）等方法，对30例胃溃疡患者愈合前后胃黏膜组织中SLPI的表达进行检测。【结果】免疫组化染色法显示，SLPI主要表达于胃黏膜上皮及胃腺上皮细胞胞质中，溃疡愈合前胃黏膜组织中SLPI的表达水平为A=0.7083±0.0593，与愈合后A=0.2641±0.0236相比明显增强（P〈0.05）（A为平均吸光度）。RTPCR检测结果显示SLPI mRNA的表达与其蛋白质的改变相一致（P〈0.05）。【结论】SLPI可能通过促进成纤维细胞和血管内皮细胞的增殖活化，促进结缔组织增生和微血管形成，从而促进溃疡愈合。

【Objective】To explore the role of secretary leukocyte protease inhibitor（SLPI）in the mechanism of peptic ulcer via investigating its expression and distribution in gastric mucosa during gastric ulcer healing. 【Methods】Expression of SLPI in gastric mucosa of 30 patients with gastric ulcer before and after treatment was examined by ABC immunohistochemistry stain and reverse transcription polymerase chain reaction （RTPCR）. 【Results】The expressions of SLPI stain were found mainly in the cytoplasm of epithelial cells in gastric mucosa. Absorbance of SPLI in gastric mucosa before treatment （A=0.7083±0.0593） was significantly higher than in the healing mucosa after treatment （A=0.2641±0.0236,P〈0.05）. Meanwhile, mRNA level change of SLPI by PTPCR demonstrated very similar findings compared to that of SLPI protein expression change（P〈0.05）. 【Conclusion】The overexpression of SLPI may be involved in gastric ulcer healing by regulating connective tissue formatting and angiogenesis.