自体骨髓间充质干细胞复合富血小板纤维蛋白促进拔牙窝骨愈合（英文）

Alveolar bone defect repair using autologous bone marrow mesenchymal stem cells combined with platelet-rich fibrin

背景:牙槽骨骨量不足将不能满足种植修复的审美和功能重建的要求。目的:观察自体第3代骨髓间充质干细胞复合富血小板纤维蛋白修复新西兰兔拔牙创窝骨缺损的效果。方法:将27只新西兰兔随机等分为模型组、富血小板纤维蛋白组和骨髓间充质干细胞/富血小板纤维蛋白组,均拔除下颌左侧中切牙。骨髓间充质干细胞/富血小板纤维蛋白组牙槽窝内植入自体骨髓间充质干细胞与富血小板纤维蛋白的复合物,富血小板纤维蛋白组牙槽窝植入富血小板纤维蛋白,模型组牙槽窝不植入任何材料。结果与结论:1模型组兔牙槽嵴及黏膜明显凹陷,宽度变窄;而富血小板纤维蛋白组和骨髓间充质干细胞/富血小板纤维蛋白组兔牙槽骨骨壁厚度、牙槽骨宽度、高度差值、骨密度增加,且骨髓间充质干细胞/富血小板纤维蛋白组牙槽骨骨壁厚度、牙槽骨宽度、高度差值、骨密度大于富血小板纤维蛋白组;骨髓间充质干细胞/富血小板纤维蛋白组骨小梁排列情况优于富血小板纤维蛋白组和模型组。2结果说明骨髓间充质干细胞复合富血小板纤维蛋白填充在拔牙窝内能够起到促进拔牙窝骨质生长,达到促进牙槽骨生长与修复的目的。

BACKGROUND： Alveolar bone deficiency will not meet aesthetic and functional requirements for dental implants. OBJECTIVE： To observe the repair effect of passage 3 autologous bone marrow mesenchymal stem cells（BMSCs） and platelet-rich fibrin（PRF） on alveolar bone defects in rabbits. METHODS： Twenty-seven New Zealand rabbits were randomly divided into BMSCs/PRF group, PRF group and model group（n=9 per group）. The left mandible incisors were extracted in all the rabbits under general anesthesia. BMSCs/PRF group was immediately implanted BMSCs/PRF composite into the alveolar socket, PRF group only implanted PRF, and model group implanted nothing. RESULTS AND CONCLUSION： In the model group, the alveolar crest and alveolar mucosa become sunken notably and narrowed. In the BMSCs/PRF and PRF groups, the thickness of alveolar bone wall, alveolar bone width, alveolar bone height difference, and bone mineral density were all increased, especially in the former group. In addition, the trabecular arrangement was better in the BMSCs/PRF groups than the model and PRF group. Our findings indicate that alveolar socket filling with composite of BMSCs and PRF can achieve preservation of alveolar bone width and height after tooth extraction in rabbits.