妇科养坤丸与骨髓间充质干细胞移植修复薄型子宫内膜

Therapeutic effect of Fukeyangkun pills and bone marrow mesenchymal stem cells transplantation on thin endometrium

背景:已有研究发现骨髓抑制后女性患者的子宫内膜中检测到来源骨髓供体的内膜细胞。目的:探讨妇科养坤丸对骨髓间充质干细胞治疗薄型子宫内膜的促进作用。方法:取处于动情期的雌性大鼠30只随机分为正常对照组、模型组、单药组、骨髓间充质干细胞组、联合组,每组6只,除正常对照组外,其余各组大鼠建立薄型子宫内膜模型。正常对照组正常喂食,模型组尾静脉注射生理盐水,骨髓间充质干细胞组造模后6 h和10 d经尾静脉注射异体骨髓间充质干细胞1 mL,单药组造模后连续20 d灌胃妇科养坤丸水溶液5 m L/kg,联合组造模后6 h和10 d尾静脉注射骨髓间充质干细胞1 m L以及连续20 d灌胃妇科养坤丸水溶液10 mL/kg。造模后第21天苏木精-伊红染色观察子宫内膜组织形态变化,测量子宫内膜厚度,免疫印迹法检测子宫内膜角蛋白与波形蛋白的表达。结果与结论:(1)与模型组相比,骨髓间充质干细胞组、单药组和联合组的子宫内膜均有不同程度增厚,其中联合组最接近正常对照组。(2)正常对照组的角蛋白和波形蛋白表达最强,模型组的表达最低,单药组、骨髓间充质干细胞组和联合组依次升高(P<0.05)。(3)结果表明骨髓间充质干细胞移植于薄型子宫内膜大鼠体内可修复子宫内膜组织,妇科养坤丸能够显著促进骨髓间充质干细胞对子宫内膜的修复作用。

BACKGROUND： Studies have found that endometrial cells from bone marrow donors can be detected in the endometrium of female patients after bone marrow suppression. OBJECTIVE： To investigate the effect of Fukeyangkun pills combined with bone marrow mesenchymal stem cells（BMSCs） transplantation in the treatment of thin endometrium. METHODS： Thirty female rats at rutting period were randomized into control, model, Fukeyangkun pills, cell transplantation and combined group（n=6 per group）. Rat models of thin endometrium were made in the latter five groups. Rats in the six groups were respectively subjected to routine feeding, tail vein injection of normal saline, tail vein injection of bone marrow mesenchymal stem cells（1 m L） at 6 hours and 10 days after modeling, intragastric administration of 5 m L/kg Fukeyangkun pill solution for continuous 20 days, or tail vein injection of bone marrow mesenchymal stem cells（1 m L） at 6 hours and 10 days after modeling plus intragastric administration of 10 m L/kg Fukeyangkun pill solution for continuous 20 days. At 21 days after modeling, hematoxylin-eosin staining was used to observe the morphological changes of the endometrical tissues and measure the endometrium thickness. The expression of cytokeratin and vimentin was determined by western blot assay. RESULTS AND CONCLUSION： Compared with the model group, the endometrium thickness and the expression of cytokeratin and vimentin（P〈0.05） were increased successively in the Fukeyangkun pills group, cell transplantation group, and combined group to different extents. Of these groups, the endometrium thickness and the expression of cytokeratin and vimentin in the combined group were the most close to normal levels. Our data demonstrate that bone marrow mesenchymal stem cell transplantation can induce regeneration of the endometrial cells and repair endometrial tissue. Furthermore, treatment of Fukeyangkun pills obviously augments the repair effect of bone marrow mesenchymal stem cell transplantation on thin endometrium.