摘要
背景:干细胞转化技术研究已成为干细胞研究的一个活跃领域,对干细胞基础研究及临床研究均产生巨大的推动作用。目的:对干细胞转化技术研究现状及进展做一综述。方法:在标题和摘要中,以"Stem cells,Transformation,differentiation"或"干细胞,转化,分化"为主题检索词,检索中国期刊全文数据库(CNKI)、PubM ed数据库中2000年1月至2015年4月关于干细胞分化技术的文章。优先选择近期发有或发表在权威杂志上的文章。初检得到121篇文章,根据纳入标准,选择其中的64篇进行综述。结果与结论:干细胞转化技术近些年来取得了较大的进展,包括改进培养液的配比,增加特定的诱导因子、蛋白质、酶、受体,注射特定的基因,使用新氧化剂,改善培养微环境,在低氧环境下培养,使用支架,采用转基因技术,三维球形培养,共培养等。干细胞转化新技术的诞生和使用,显著提高了干细胞的转化率,为人类难治性疾病的治疗带来希望。
BACKGROUND： Stem cell conversion technology has become an active area in the field of stem cell research, and it also has a tremendous boost on both the stem cell basic research and clinical study. OBJECTIVE： To review the research status and the current progress of stem cell conversion technology. METHODS： In the titles and summaries, the ＂stem cells, conversion, differentiation＂ in Chinese and English served as the search terms to search articles related to stem cells technology published from January 2000 to April 2015 in CNKI and Pub Med databases. The latest articles or articles published on the authoritative journalswere preferred. Totally 121 articles were obtained after initial survey, and according to the inclusion criteria, 64 articles were selected for overview. RESULTS AND CONCLUSION： The progress of stem cells conversion technology has driven the development of the entire stem cell research, including modifying the ratio of culture medium, increasing specific inducers, proteins, enzymes or receptors, injecting specific genes, using new oxidizing agents, modifying culture microenvironment, culture under hypoxia, scaffold usage, applying transgenic technology, three-dimensional spherical culture or co-culture. Stem cell conversion technology will definitely increase the conversion rate of stem cells, which can bring hope for the treatment of refractory diseases.
出处
《中国组织工程研究》
CAS
北大核心
2016年第1期128-134,共7页
Chinese Journal of Tissue Engineering Research
