An UPLC-MS/MS application to investigate chemical compositions in the ethanol extract with hypoglycemic activity from Zingiber striolatum Diels

阳荷醇提物降血糖活性及其化学成分的UPLC-MS/MS研究（英文）

Zingiber striolatum Diels （Zingiberaceae） is an edible plant resource in the Chinese folk with special efficacy in relieving diabetes and constipation, which has been documented in the Compendium ofMateria Medica. However, its hypoglycemic activity and constituents have not been reported yet. In the present study, we evaluated the hypoglycemic activity of Z. striolatum in insulin-resistant HepG2 cells, and we developed ultra performance liquid chromatography coupled with tandem mass spectrometry （UPLC-MS/MS） based chemical profiling method for rapid analysis of Z. striolatum. As a result, the ethanol extract from Z. striolatum showed significant hypoglycemic activity in HepG2 cells, and 22 flavonoids compounds were tentatively characterized by comparing the retention time and mass spectrometry data. In conclusion, the method of hypoglycemic screening in insulin-resistant HepG2 cells coupled with UPLC-MS/MS is a feasible and credible technique to separate and identify the active constituents in complex matrices of traditional Chinese medicine.

阳荷(姜科)是一种药食同源的植物,据《本草纲目》记载,阳荷对治疗糖尿病和便秘有特效,但其降血糖活性和化学成分的系统分析还未见报道。在本研究中,将阳荷粗提物作用于胰岛素抵抗的HepG2细胞,用葡萄糖试剂盒检测其降血糖活性,MTT法检测细胞活力,并应用超高效液相色谱串联质谱法(UPLC-MS/MS)来快速分析阳荷中的化学成分。结果显示阳荷粗提物在HepG2细胞中具有显著的降血糖活性,并通过比较保留时间、质谱数据及参考文献推定出22个黄酮类化合物。结果表明这是一种可行且可靠的分离和鉴定中药活性成分的方法。