蜂王浆酶解产物对D-半乳糖模型小鼠体内抗衰老的作用

The in Vivo Antiaging Effect of Enzymatic Hydrolysate from Royal Jelly in D-galactose Induced Aging Mouse

通过构建D-半乳糖诱导的衰老小鼠模型,检测蜂王浆酶解产物对衰老模型小鼠外部衰老表征和内部衰老表征的改善作用,全面衡量蜂王浆酶解产物的体内抗衰老活性。结果表明,在改善外部衰老方面,低剂量的蜂王浆酶解产物能够干预D-半乳糖诱导的小鼠体质量下降的状况。高、低剂量的蜂王浆酶解产物均能改善衰老小鼠活动能力下降的情况。同时,蜂王浆酶解产物还能够提高小鼠非空间性和空间性长期学习记忆能力,使之保持良好的记忆力,对空间和方位的快速判断力以及对学习和训练结果的巩固能力,具有优异的回避危害的学习能力及长期记忆能力。在改善内部衰老方面,蜂王浆酶解产物能延缓胸腺等器官的萎缩,表现出对器官衰老的抑制作用。蜂王浆酶解产物还能够提高小鼠血清超氧化物歧化酶、小鼠肝脏过氧化氢酶、小鼠肝脏谷胱甘肽过氧化物酶的活力并抑制小鼠脑中丙二醛的积累,高剂量效果等同或显著优于蜂王浆。

The antiaging activity of the RJ hydrolysate was evaluated using the D-galactose induced aging mouse model in vivo. In the respects of improving external aging performance, low dose of RJ hydrolysate could significantly prevent the body weight loss of the aging mouse. Both low and high doses of RJ hydrolysate improved the age-related locomotor decline. Moreover, the administration of RJ hydrolysate improved both spatial and non-spatial long term memory of mice, help them maintain good spatial orientation judgment, excellent learning and memory about the training after a long period of time. In the respects of improving internal aging performance, given RJ hydrolysate help to delay the atrophy of thymus to prevent immune function diminished with aging. RJ hydrolysate also increased antioxidant enzyme activity and inhibit the production of lipid peroxides of mice, which was equal to or even better than RJ did.