产果胶酶菌株——黑曲霉的复合诱变及产酶条件研究

Study on the Compound Mutation and Pectinase-producing Conditions of Aspergillus niger

以产果胶酶菌株——黑曲霉YQ-13为出发菌株,通过紫外与氯化锂复合诱变反复处理,最终获得1株能够稳定遗传的突变菌株YQY-36,其果胶酶活力为67.6 U/m L,比出发菌株提高了2.44倍。应用PlackettBurrman设计筛选主要影响因素,通过最陡爬坡试验确定中心点后用响应面法对突变菌株YQY-36的发酵培养基进行优化。优化后培养基成分(g/L)是:桔皮粉42.5,葡萄糖5.0,蛋白胨28.5,(NH_4)_2SO_415.0,Na_2HPO_4·12H_2O 1.7,KH_2PO_41.4,Zn SO_4·7H_2O 0.5。优化培养基后果胶酶活力达92.1 U/m L,比优化前提高了36.2%。

A pectinase-producing strain——Aspergillus niger YQ-13 was mutagenized with UV-Li Cl and the mutant YQY-36 was obtained. The pectinase activity of the mutant YQY-36 was increased to 67.6 U/mL, 2.44 times higher than that of the original strain YQ-13. Plackett-Burman, steepest ascent and central composite designs were employed to optimize the fermentation media of YQY-36 in this study. The results showed that the optimum medium contained（g/L）：orange peel 42.5, glucose 5.0, peptone 28.5,（NH4）2SO415.0, Na2HPO4·12H2O 1.7, KH2PO41.4, Zn SO4·7H2O 0.5. As a result, the maximum pectinase activity of strain YQY-36 was 92.1 U/mL and increased 36.2% after optimization.