壮族人群肠道菌群多样性分析

The Diversity of Gut Microbiota of Zhuang Ethnic Group Living in Urban and Rural

以中国广西壮族自治区生活在城市和乡村的46位健康成年壮族人为研究对象,分析城乡壮族人群肠道菌群的多样性。通过PCR-DGGE图谱的PCA分析,结果表明:城乡壮族人群肠道菌群结构按照实际分组情况明显的分为两簇;16S r RNA V3区的测序结果显示城乡壮族人群肠道菌群都以厚壁菌门、拟杆菌门、变形菌门和放线菌门为主;城市壮族人群以志贺氏菌属(Shigella)和假双歧杆菌为特有菌株,而乡村壮族人群肠道以Parabacteroides gold-steinii,乳杆菌属和动物双歧杆菌为特有菌株;尽管两组中SCFA含量无显著性差异,但乡村壮族人群肠道内的乳杆菌属和双歧杆菌属的多样性明显区别于城市壮族人群。综上所述,在不同膳食结构和生活习惯下,城乡壮族人群肠道菌群的多样性存在明显差异。

This study focused ongut microbiota of Zhuang ethnic group living in ru-ral and urban. Total of 46 volunteers from rural and urban were recruitedwith different dietary patter and life-style, seeking the diversity of structural gutmicrobiota be-tween these two groups.PCA was performed on the base of PCR-DGGE profile, showingsamples were divided into two groups as urban and rural groups. With 16 S r RNA V3 sequencing were done, the results showed that Firmicutes, Bacteroidetes, Proteobacteria and, Actinobacteria are predominant. DGGE profile of Shigella sp. and Bifidobacterium pseudocatenulatum were predominant in the gut of urban group, besides Parabacteroides goldsteini, Lactobacillus sp. and Bifidobacterium animaliin the gut of urban group. The SCFA content was no statistical differences between two groups. The diversity of Lactobacillus and Bifidobacterium of the rural group was differect from that of the urban group. These gregation of structural gut microbiota was discovered between urban and rural groups. For the diversity of Lactobacillus and Bifidobacterium, these two groups have obviously differences. The different dietary patter and life-stylemay cause these differences of structural gut microbiota between two groups.