微RNA基因多态性与食管癌患者急性放射性食管炎易感性及预后的关系

Correlation between microRNA polymorphism and the risk and clinical prognosis of acute radiation esophagitis in patients with esophageal neoplasms

目的：探讨微RNA（miRNA）前体区域基因单核苷酸多态性（SNP）与食管癌患者急性放射性食管炎易感性和预后的关系。方法采用病例对照研究设计，选取放疗中出现急性放射性食管炎的256例食管癌患者作为病例组，按性别和年龄匹配的未出现急性放射性食管炎的256例食管癌患者作为对照组。采用ABI7900HT的Taqman基因分型技术检测miRNA-146a多态位点rs2910164的基因型，计算miRNA-146a多态位点rs2910164基因类型在病例组和对照组中的分布情况。采用Logistic回归计算校正相对危险度（OR）和95%置信区间（95%CI），评价基因型对急性放射性食管炎易感性的影响。结果 miRNA-146a多态位点rs2910164基因类型CC、GG和CG在对照组和病例组患者中的分布频率分别为20.70%（53／256）和33.20%（85／256）、45.32%（116／256）和40.63%（104／256）、33.98%（87／256）和26.17%（67／256），差异均具有统计学意义（均P＜0.05）。与基因型CC比较，基因型GG和CG患者发生急性放射性食管炎的OR值分别为0.654和0.627（P＜0.05），可降低发病风险。病例组中基因型GG、CG和CC患者的急性放射性食管炎治疗无效率分别为7.69%（8／104）、19.40%（13／67）和41.18%（35／85），各基因型的临床预后差异具有统计学意义（P＜0.05）。结论miRNA-146a多态位点rs2910164的CC基因型可增加食管癌患者发生急性放射性食管炎的风险，并且降低急性放射性食管炎的临床预后。

Objective To study the correlation between microRNA （miRNA） polymorphism and the risk and clinical prognosis of acute radiation esophagitis in patients with esophageal neoplasms. Methods 256 patients with acute radiation esophagitis during radiotherapy were chosen as the experimental group, and 256 patients matched by age and sex without acute radiation esophagitis during radiotherapy were chosen as the control group. The polymorphism types of miRNA-146a （rs2910164） were determined by Taqman gene typing technology of ABI7900HT. The genotype distribution of miRNA-146a rs2910164 polymorphism in the experimental and control groups was analyzed. Logistic regression was performed to estimate the odds ratio （OR） and 95%confidence interval （95%CI ）. Results The genotype frequencies of CC, GG and CG at miRNA-146a polymorphic site rs2910164 in the experiment and control group were 20.70 % （53/256） and 33.20 % （85/256）, 45.32 % （116/256） and 40.63 % （104/256）, 33.98 % （87/256） and 26.17 % （67/256）, respectively. There was a statistically significant difference between two groups （all P〈 0.05）. Compared with gene type CC, the OR values of acute radiation esophagitis in patients with gene type GC and GG were 0.654＆amp;nbsp;and 0.627, respectively （P〈 0.05）, indicating that they had a low risk. The negative effect rates in patients with gene type GG, CG and CC were 7.69 % （8/104）, 19.40 % （13/67） and 41.18 % （35/85）, respectively. There was a statistically significant difference in the clinical prognosis among these genotypes （P〈 0.05）. Conclusion Gene type CC at miRNA-146a polymorphic site rs2910164 can increase the risk of acute radiation esophagitis and decrease the clinical prognosis in patients with esophageal neoplasms.