VEGF基因多态性及吸烟在诱发非酒精性脂肪肝中的交互作用

Gene polymorphism of VEGF and its interaction with smoking in patients with non-alcoholic fatty liver disease

目的观察非酒精性脂肪肝(NAFLD)患者血管内皮生长因子(VEGF)基因rs833061、rs3025039位点多态性,并探讨其多态性及吸烟在诱发NAFLD中的交互作用。方法选择NAFLD患者341例(NAFLD组)、健康体检者246例(对照组),采用PCR-RFLP法分析VEGF基因rs833061、rs3025039位点基因型,非条件Logistic回归分析法分析各基因型与NAFLD易感性的关系,非条件Logistic回归分析法和广义多因子降维法分析VEGF基因多态性及吸烟在诱发NAFLD中的交互作用。结果 VEGF基因rs833061位点C基因携带者(CC+CT)罹患NAFLD的风险较非C基因携带者(TT)升高(P均<0.01),rs3025039位点T基因携带者(TT+CT)患病风险较非T基因携带者(CC)风险升高(P均<0.01)。VEGF基因rs833061位点多态性与吸烟可能在NAFLD发病过程中存在相加作用(P<0.01),rs833061位点CC/CT基因型吸烟个体罹患NAFLD风险是携带TT基因型非吸烟个体的4.93倍。结论VEGF基因多态性与NAFLD发病有关,VEGF基因rs833061位点多态性与吸烟在诱发NAFLD过程中具有协同效应。

Objective To observe the gene polymorphisms of vascular endothelial growth factor（ VEGF） rs833061 and rs3025039 and to investigate their interaction with smoking in patients with non-alcoholic fatty liver disease（ NAFLD）.Methods Genotypes of VEGF rs833061 and rs3025039 in 341 patients with NAFLD（ NAFLD group） and 246 control subjects（ control group） were examined by polymerase chain reaction-based restriction fragment length polymorphism（ PCR-RFLP）. The unconditional logistic regression（ ULR） was performed to analyze the relationships between the genotypes and susceptibility to NAFLD. The interactions between genotypes of VEGF rs833061 and rs3025039 and smoking in inducing NAFLD were explored by generalized multifactor dimensionality reduction（ GMDR） and unconditional Logistic regression method. Results The risk of NAFLD in VEGF rs833061 with genotype C（ CC ＋ CT） was higher than that of carriers without C（ TT）（ all P 0. 01）,and the risk of NAFLD in rs3025039 T gene carriers（ TT ＋ CT） was higher than that of carriers without genotype T（ CC）（ all P 0. 01）. Gene polymorphism of VEGF rs833061 had interactions with smoking in the process of NAFLD（ P 0. 01）. The risk of NAFLD in rs833061 CC / CT genotype of smoking individuals was 4. 93 times higher than that of TT genotype of non-smoking individuals. Conclusions VEGF gene polymorphism is associated with the risk of NAFLD. VEGF rs833061 and smoking have synergistic effects in the process of inducing NAFLD.