SCI大鼠脊髓前角细胞自噬相关蛋白Beclin-1表达的变化

CHANGES OF AUTOPHAGY RELATED PROTEIN BECLIN-1 EXPRESSION IN ANTERIOR HORN CELLS OF SPINAL CORD INJURY RATS

目的:观察脊髓全横断损伤对脊髓前角细胞Beclin-1表达的影响。方法:雄性Wistar大鼠随机分为模型组和假手术组,建立大鼠第2腰髓全横断损伤模型,分别于损伤后6h、1d、3d、7d、14d、21d取3-4腰髓,采用免疫荧光染色法观察大鼠脊髓前角细胞自噬相关蛋白Beclin-1的表达情况。结果:与假手术组相比,模型组损伤后各时间点大鼠脊髓前角Beclin-1免疫阳性细胞数明显升高(P<0.01,P<0.05)。脊髓损伤后3d脊髓前角细胞Beclin-1免疫阳性细胞数达到高峰,21d最低。结论:Beclin-1可能参与了神经元自噬状态的改变。

Objective： To observe the effects of complete transection of spinal cord on Beclin-1 expression in anterior hom cells of rats＇ spinal cord. Methods： Male Wistar rats were randomly divided into model group and shamoperation group. The spinal cord injury （SCI） rat＇s model were made by completely Wansecting the 2^nd lumbar spinal cord. Immunofluorescent staining was used to detect the Beclin-1 protein expression in anterior horn cells of the 3^rd-4^th lumbar spinal cord 6h, 1d, 3d, 7d, 14d and 21d after injury respectively. Results： Compared with rats in sham-operation group, the Beclin- 1 positive cells in anterior hom of spinal cord of rats in model group at each time point increased obviously （P〈 0.01, P〈 0.05）. The Beclin-1 positive cells in anterior hom of spinal cord reached the peak 3 days after injury, and reached the lowest 21 days after injury. Conclusions： Beclin- 1 may be involved in the changes of neuron autophagy.