摘要
目的:观察脊髓全横断损伤对脊髓前角细胞Beclin-1表达的影响。方法:雄性Wistar大鼠随机分为模型组和假手术组,建立大鼠第2腰髓全横断损伤模型,分别于损伤后6h、1d、3d、7d、14d、21d取3-4腰髓,采用免疫荧光染色法观察大鼠脊髓前角细胞自噬相关蛋白Beclin-1的表达情况。结果:与假手术组相比,模型组损伤后各时间点大鼠脊髓前角Beclin-1免疫阳性细胞数明显升高(P<0.01,P<0.05)。脊髓损伤后3d脊髓前角细胞Beclin-1免疫阳性细胞数达到高峰,21d最低。结论:Beclin-1可能参与了神经元自噬状态的改变。
Objective: To observe the effects of complete transection of spinal cord on Beclin-1 expression in anterior hom cells of rats' spinal cord. Methods: Male Wistar rats were randomly divided into model group and shamoperation group. The spinal cord injury (SCI) rat's model were made by completely Wansecting the 2^nd lumbar spinal cord. Immunofluorescent staining was used to detect the Beclin-1 protein expression in anterior horn cells of the 3^rd-4^th lumbar spinal cord 6h, 1d, 3d, 7d, 14d and 21d after injury respectively. Results: Compared with rats in sham-operation group, the Beclin- 1 positive cells in anterior hom of spinal cord of rats in model group at each time point increased obviously (P〈 0.01, P〈 0.05). The Beclin-1 positive cells in anterior hom of spinal cord reached the peak 3 days after injury, and reached the lowest 21 days after injury. Conclusions: Beclin- 1 may be involved in the changes of neuron autophagy.
出处
《承德医学院学报》
2016年第2期102-104,共3页
Journal of Chengde Medical University
基金
河北省卫生厅重点课题(20150016)
河北省中医药管理局课题(2015153)