HIV-1对人精子IGF2和MEST基因甲基化及其表达的影响

Research on the influence of HIV-1 infection in the methylation and expression of genetic imprinted genes IGF2 and MEST

目的探讨HIV-1对感染者精子中IGF2和MEST基因甲基化及其表达的影响。方法采集HIV-1感染者精液,采用重亚硫酸盐测序法和实时荧光定量PCR(Real time-PCR)检测IGF2和MEST基因启动子甲基化状态及m RNA表达。结果与对照组比较,HIV实验组IGF2 m RNA表达水平略高于对照组,但两者差异比较无统计学意义(P=0.120);HIV实验组MEST m RNA表达水平低于对照组,两者差异比较有统计学意义(P=0.002);HIV实验组IGF2基因甲基化频率为(96.63±3.37)%,对照组甲基化频率为(96.02±1.84)%,两者比较无统计学差异意义(P=0.711);HIV实验组MEST基因甲基化频率为(1.29±1.04)%,对照组为(1.46±0.89)%,HIV实验组甲基化频率与对照组比较,差异无统计学意义(P=0.751)。结论在HIV实验组与对照组中检测IGF2、MEST启动子区甲基化状态均无差异。两组之间IGF2 m RNA也无差异,MEST m RNA表达在HIV实验组和对照组间存在显著差异,可能是HIV病人精液质量下降的一个原因。

Objective：To investigate the influence of HIV-1 in the methylation status and m RNA expression of genetic imprinted genes IGF2 and MEST. Methods：The methylation status and m RNA expression of imprinted gene IGF2 and MEST were tested by bisulfite sequencing and realtime-PCR,respectively. Results：The m RNA expression of IGF2 in HIV-infected group was higher than that in healthy group,but the difference was not significantly（P=0.949）. The m RNA expression of MEST in HIV-infected group was higher than that in healthy group,and it showed significantly difference（P=0.002）. The frequency of methylation of IGF2 in HIV-infected group was higher than that in healthy group,but it did not show significantly difference（P=0.711）. The frequency of methylation of MEST in HIV-infected group was lower than that in healthy group,which did not showed significantly difference（P=0.751）. Conclusions：There was no significant difference for the methylation status and m RNA expression of IGF2 promoter region between HIV-1 infected group and healthy group,and the no significant difference for methylation status of MEST promoter region between HIV-1 infected group and healthy group,However,m RNA expression status of MEST between HIV-1 infected group and healthy group were significant,suggesting that expression of MEST of MEST m RNA maybe a reason of decreased semen quality in patients with HIV.