响应面法优化亚临界R134a萃取牡蛎中多环芳烃工艺

Optimization of sub-critical R134a extraction of polycyclic aromatic hydrocarbons(PAHs) from Oysters by response surface methodology

目的建立一种适用于牡蛎中痕量多环芳烃(polycyclic aromatic hydrocarbons,PAHs)萃取分析的快速、绿色、安全的新技术。方法以新鲜牡蛎作为空白样品基质,以US EPA 16PAHs的加标回收率为实验指标,利用单因素和响应曲面实验,优化亚临界R134a(1,1,1,2-四氟乙烷)萃取牡蛎中PAHs工艺。结果亚临界R134a萃取PAHs的最佳工艺条件为:萃取压力6.21 MPa,萃取温度42.7℃,夹带剂用量3.39 m L。在此工艺条件下,16种PAHs的平均加标回收率为115.9%。结论本研究可为实际样品中PAHs的萃取分析提供指导。

Objective To establish a fast, green and safe technology for the extraction and analysis of trace polycyclic aromatic hydrocarbons（PAHs） from oysters. Methods With fresh oysters as blank sample matrix, the US EPA 16 PAHs standard addition recovery as experimental indexes, the process of sub-critical R134 a extraction of PAHs was optimized. Results The optimum conditions were extraction pressure 6.21 MPa, extraction temperature 42.7 ℃, and co-solvent amount 3.39 m L. Under these conditions, the average spiked recoveries of 16 kinds of PAHs were 115.9%. Conclusion This study can provide guidance for the extraction and analysis of PAHs in actual samples.