放射免疫法快速检测牛血清中磺胺类药物

Detection of sulfonamides in bovine serum by radioimmunoassay method

目的应用Charm Ⅱ放射免疫分析方法检测牛血清中磺胺类药物残留,以便能大大提高检测速度,快速高效地完成磺胺类药物检测。方法在活体家畜上采集血清样品进行检测。确定控制点设定的方法,采集6个牛血清样品进行添加检测,设定控制点,样品结果与控制点比较。评价免疫反应体系的灵敏度和特异性。测得数值与控制点比较,测的数值大于控制点即为阴性,反之测得数值小于控制点即为阳性样品。结果磺胺类检测限可达20μg/kg,短时间可出检测结果,此方法特异性强,与除磺胺类药物外的其他药物无交叉反应。结论此方法快速,简便。虽然此方法只是初筛方法,测得阳性结果时则需采用其他检测方式进一步确证。但已经大大提高了检测效率,既保持了家畜的鲜活状态,又能快速得到检测结果,大大加快通关速度,促进了我国的活畜出口量。

Objective To detect the sulfonamide residues in bovine serum by Charm Ⅱ radioimmunoassay method, so as to improve the detection rate greatly and complete the sulfa drug testing quickly and efficiently. Methods Serum samples which collected from the live livestock were detected. The determination method of control point was made. Six serum samples were collected for the addition test. The control point was set. The results from the sample test were compared with the control point. The sensitivity and specificity of the immune reaction system was evaluated. The measured value was compared with the control point. The sample was determined to be negative if the measured value was greater than the control point, whereas, the sample was positive if the measured value was less than the control point. Results The limit of sulfonamide detection was 20 μg/kg. The whole detection process could be finished within short time. This method had strong specificity and showed no cross reactions with drugs other than sulfonamides. Conclusion This method is rapid and convenient. Although other test methods are still needed to further confirm the positive results as the method proposed in this paper is only a preliminary method, the method improves test efficiency and can both keep livestock alive and quickly provide the test results, thus greatly speeding up customs clearance and increasing livestock export volume of our country.