4种野生菌子实体乙醇提取物体外抗氧化活性的研究

Research on in vitro antioxidant activity of ethanol extracts from 4 kinds of wild fungi

目的研究柠檬黄蜡伞(Hygrophorus lucorum)、马鞍菌(Helvella elastica)、绒边乳菇(Lactarius pubescens)、晶粒鬼伞(Coprinus micaceus)4种野生菌子实体的体外抗氧化能力。方法在分别测定4种乙醇提取物总酚、总黄酮和粗三萜含量的基础上,采用清除DPPH自由基、抑制小鼠肝组织脂质过氧化及对Fe^(2+)的螯合能力实验,分别检测提取物的体外抗氧化能力。结果柠檬黄蜡伞、马鞍菌、绒边乳菇抗氧化活性较好,优于晶粒鬼伞。4种子实体乙醇提取物对DPPH均有一定的清除能力。除晶粒鬼伞外,其余三种在乙醇提取物为10 mg/m L时,对DPPH清除率在85%以上。柠檬黄蜡伞在浓度大于0.5 mg/m L时,小白鼠肝脂质过氧化的抑制率高于阳性对照BHA。4种子实体提取物均有一定的金属离子螯合能力,呈现剂量效应关系。结论野生菌的抗氧化活性与总酚、粗三萜含量相关;柠檬黄蜡伞、绒边乳菇、马鞍菌具有较好的体外抗氧化活性,可作为提取天然抗氧化剂的原料。

Objective To investigate the in vitro antioxidant activity of Hygrophorus lucorum, Helvella elastica, Lactarius pubescens and Coprinus micaceus. Methods Based on the determination of total phenol, total flavonoids and crude terpene of four kinds of mushroom ethanol extracts, DPPH radical scavenging experiment, mice liver tissue in vitro spontaneous lipid peroxidation experiment and Fe^（2＋） chelating experiment were carried out to detect the antioxidant activity. Results The antioxidant activity of Hygrophorus lucorum, Helvella elastica, Lactarius pubescens were better than that of Coprinus micaceus. Four kinds of mushroom ethanol extracts had certain antioxidant activity against DPPH. The radical-scavenging ability was more than 85% when the mushroom ethanol extract reached to 10 mg/m L except for the Coprinus micaceus. The inhibition of lipid peroxidation activity was higher than that of the positive control BHA when the concentration of Hygrophorus lucorum was more than 0.5 mg/m L. Fe^（2＋） chelating experiment showed that the four kinds of mushroom ethanol extracts all had the ability of chelating Fe^（2＋）. Conclusion The antioxidant activity of fungi is related to total phenolics and total flavonoids. Hygrophorus lucorum, Helvella elastica, Lactarius pubescens have good antioxidant activity, which will provide a theoretical basis for the rational development of large fungi.