基于液相色谱-四极杆飞行时间质谱产莫能菌素芽胞杆菌筛选

Screening of Bacillus strains producing monensin A based on liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry

目的筛选产莫能菌素的芽胞杆菌菌株。方法采用液相-四级杆飞行时间质谱的方法结合Metlin数据库检索分析35株芽胞杆菌菌株的发酵液胞外成分。运用安捷伦公司的Mass Hunter软件,对数据进行分子特征提取,并通过Metlin代谢物质谱数据库检索,获得总体代谢物的信息。结果筛选出含莫能菌素的芽胞杆菌菌株为波茨坦短芽胞杆菌(Brevibacillus borstelensis)FJAT-10006。其中,莫能菌素的相对含量占其发酵液总代谢物的1.20%,其匹配得分达到93.75,保留时间为27.9518 min,精确质量数为670.4308。结论波茨坦短芽胞杆菌(Brevibacillus borstelensis)FJAT-10006为产莫能菌素菌株。为波茨坦短芽胞杆菌来源的抗真菌抗生素的开发与利用提供理论依据。

Objective To screen the Bacillus strains producing monensin A. Methods Extracellular metabolites of 35 Bacillus strains were analyzed by liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry（LC-QTOF-MS）. Molecular feature extraction（MFE） and database retrieval were applied by Agilent Mass Hunter software, for the information overall metabolites. Results The results showed that only one Bacillus strain, Brevibacillus borstelensis FJAT-10006, contained monensin A. Monensin A was detected with the match score of 93.75, and relative content of 1.20%. The retention time of monensin A was about 27.9518 min, and accurate mass was 670.4308. Conclusion Brevibacillus borstelensis FJAT-10006 is a Bacillus strain producing monensin A. The results provide the theory basis for development and utilization of antifungal antibiotic from Bacillus strains.