气相色谱-质谱联用法分析罗布麻蜂蜜中脂肪酸

Determination of fatty acids in Apocynum honey by gas chromatography-mass spectrometry

目的建立气相色谱-质谱联用法快速测定脂肪酸的方法,分析新疆罗布麻蜂蜜中组成成分。方法样品用正己烷乙醚混合提取,氢氧化钾-甲醇法甲酯化,归一化法计算含量。结果罗布麻蜂蜜中含有28种脂肪酸成分,其主要成分是亚油酸(26.57%)、棕榈酸(17.6%)、十七烷酸(7.34%)等。饱和脂肪酸含量为38.83%,不饱和脂肪酸为61.17%。结论罗布麻蜂蜜中不饱和脂肪酸含量丰富,可为罗布麻产品的开发利用提供依据,该方法简便快速,适合于大批量样品的测定。

Objective To establish a method for determination of fatty acids in Apocynum honey by gas chromatography-mass spectrometry（GC-MS）. Methods Fatty acids extracted by the mixture of hexane and aether were converted to methyl esters with KOH-CH_3OH. Then the methyl esters were determined by the area normalizing method. Result There were 28 kinds of fatty acids in Apocynum honey, with the main components of linoleic acid（26.57%）, palmitic acid（17.6%）, and heptadecanoic acid（7.34%）. The content of saturated fatty acid and polyunsaturated fatty acid were 38.83% and 61.17%, respectively. Conclusion Apocynum honey was rich in polyunsaturated fatty acid. This study provides scientific basis for exploitation and utilization of Apocynum honey.