昆阆牌加参灵片对小鼠睡眠的改善作用研究

The amelioration effects of JIASHENLING tablet on sleep in mice

目的观察昆阆牌加参灵片对小鼠睡眠的改善作用。方法将清洁级ICR雄性小鼠随机分为对照组及昆阆牌加参灵片低、中、高剂量组,每组20只。对照组灌胃给予0.5%羧甲基纤维素钠20m L/kg,昆阆牌加参灵片低、中、高剂量组分别灌胃给予昆阆牌加参灵片0.25、0.5、1.0g/kg,每日1次,连续灌胃30天。观察小鼠直接睡眠的入睡动物数及睡眠时间,戊巴比妥钠阈下剂量催眠的睡眠发生率,戊巴比妥钠阈剂量催眠的睡眠时间及巴比妥钠诱导小鼠睡眠潜伏期。结果昆阆牌加参灵片可明显延长戊巴比妥钠诱导的小鼠睡眠时间,缩短巴比妥钠诱导的小鼠睡眠潜伏期,提高戊巴比妥钠诱导的小鼠睡眠发生率,对直接睡眠小鼠入睡动物数及睡眠时间均无明显影响。结论昆阆牌加参灵片具有改善睡眠作用。

Objective To observe the amelioration effects of JIASHENLING tablet on sleep in mice. Methods Healthy ICR mice were randomly divided into control group, and low, middle and high groups of JIASHENLING tablet. Control group was treated by 0.5% CMC-Na 20 m L/kg with intragastric daministration, low, middle and high groups of JIASHENLING tablet were treated by JIASHENLING tablet 0.25, 0.5 and 1.0g/kg with intragastric daministration. One time every day,continuous intragastric daministration for 30 days.The effect of sleep improvement was studied by the expeximents of sleep-induced directly, prolonging sleeping time induced by pentobarbital sodium, affecting mice sleeping rate under the threshold hypnogenesis dosage of pentobarbital sodium and affecting sleeping deliteseenee induced by tobarbital sodium. Results JIASHENLING tablet could significantly prolong the time of sleep induced by pentobarbital sodium, shorten sleep latent period induced by barbital sodium, and boost sleep incidence rate induced by subthreshold dose of pentobm＇bital sodium, but had no significant effects on the direct sleep of mice. Conclusion JIASHENLING tablet could improve the sleep function of mice.