宁波-舟山港口航道3种致病性细菌毒力特征与耐药性

Virulence characteristics and antimicrobial susceptibilityof three pathogenic bacteria isolated from the channels and ports in Ningbo and Zhoushan

本文调查分析了宁波-舟山港口航道副溶血弧菌Vibrio parahemolyticus、溶藻弧菌Vibrio alginolyticus和嗜水气单胞菌Aeromonas hydrophila的毒力特征和耐药性特点。用PCR方法检测分离菌株的毒力基因分布模式发现,副溶血弧菌毒力基因型以gyrB^+,toxR^+,tlh^+和tdh^+为代表,溶藻弧菌毒力基因型以aspA^+,tlh^+,collagenase^+和toxS^+为代表,嗜水气单胞菌毒力基因型以aerA^+,alt^+,aha1^+和hlyA+为代表;副溶血弧菌、溶藻弧菌和嗜水气单胞菌代表菌株相应基因的检出率分别为57.1%、40.0%和50.0%。用24种常见抗生素对3种细菌的分离代表菌株进行药敏调查,耐药率分别为4.17%、8.33%和29.17%。实验结果表明宁波-舟山港口航道3种致病性细菌具备较丰富的毒力基因种类和耐药性,需要有针对性地制定预防措施。此外,鉴于毒力基因型与致病性之间的相关性,毒力基因可作为标记基因,用于致病性细菌的检测。

Virulence characteristics and antimicrobial susceptibility of Vibrio parahaemolyticus（VP）,Vibrio alginolyticus（VA）and Aeromonas hydrophila（AH）isolated from the channels and ports in Ningbo and Zhoushan were investigated.The distribution patterns of virulent genes in isolated pathogenic bacteria are found that VP is represented by tlh^＋,toxR^＋,gyrB^＋ and tdh^＋;VA is represented by aspA^＋,tlh^＋,collagenase＋and toxS^＋;AH is represented by aerA^＋,alt^＋,aha1^＋ and hlyA^＋.The detection rates of relevant genes in representative strains for these three kinds of bacteria are 57.1%,40.0%,50.0%.24 common kinds of drug resistance rates of representative strains are 4.17%,8.33%and 29.17%respectively.The results show that harmful characteristics of the pathogenic bacteria are found in Ningbo-Zhoushan,and the right defense measures needed to make to prevent them.Moreover,the correlation of virulence genotypes and pathogenicity of the bacteria show that virulent genes can be chosen as gene-markers to detect pathogenic strains of bacteria.