CD14启动子区遗传变异对喉癌遗传易感性的影响

Effect of CD14 promoter variants on the susceptibility to laryngeal cancer

目的 探讨CD14基因启动子区-260C＞T、-651C ＞T遗传变异与喉癌遗传易感性之间的关系.方法 应用病例-对照研究,选取喉癌患者163例及健康对照者326名,应用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）的方法检测CD14基因-260C＞T（rs2569190）和-651C ＞T（rs5744455）多态性位点的基因型.采用多变量Logistic回归方法计算比值比（OR）和95％可信区间（CI）.结果 与CD14-651CC基因型携带者相比,-651TT基因型携带者发生喉癌的风险显著升高,其OR值为5.79（95％ CI： 2.38 ～ 14.11,P＜0.001）.吸烟分层分析显示,在不吸烟人群中,-651TT基因型携带者喉癌发病风险的OR值为8.64（95％ CI： 1.88～39.78,P=0.006）;在吸烟人群中,-651TT基因型携带者喉癌发病风险的OR值为4.74（95％CI： 1.69 ～ 13.25,P=0.003）;此外,在轻度吸烟者和重度吸烟者中-651TT基因型携带者喉癌发病风险的OR值分别为5.40 （95％ CI：1.10 ～26.45,P=0.037）和4.30（95％CI：1.10～ 16.75,P=0.036）.饮酒分层分析显示,在不饮酒人群中,-651Tr基因型携带者喉癌发病风险的OR值为6.48（95％ CI：2.81～14.95,P＜0.001）;在饮酒人群中其OR值为2.01（95％CI： 0.65 ～6.26,P=0.227）.而CD14-260C＞T各基因型分布在正常人群和喉癌患者中差异无统计学意义（P＞0.05）.结论 CD14-651C ＞T遗传变异与喉癌遗传易感性相关.

Objective To investigate the effects of-260C 〉 T and-651C 〉 T genetic variants in the promoter region of CD14 on the susceptibility to laryngeal cancer.Methods A total of 163 patients with laryngeal cancer and 326 healthy subjects as controls were included.Genotypes of CD14-260C 〉 T （rs2569190） and-651 C 〉 T （rs5744455） variants were determined by polymerase chain reaction-based restriction fragment length polymorphism （PCR-RFLP）.Odd ratios （OR） and 95% confidence intervals （95% CI） were calculated with logistic regression analysis.Results Compared with CD14-651CC genotype carriers,-651TT genotype carriers had the increased risk of laryngeal cancer with the OR （95% CI） of 5.79 （2.38-14.11）.When the-651TF genotype carriers were stratified by smoking status, the OR （95 % CI） for laryngeal cancer was 8.64 （1.88-39.77） in nonsmokers and 4.74 （1.69-13.25） in smokers;the OR （95% CI） was 5.40 （1.10-26.45） in the light smokers and 4.30 （1.10-16.75） in the hevey smokers.When the-651TF genotype carriers were stratified by drinking status, the OR （95% CI） for laryngeal cancer was 6.48 （2.81-14.95） in nondrinkers and 2.01 （0.65-6.26） in drinkers.There was no significant difference in CD14-260C 〉 T genotype distribution between patients and controls.Conclusion Polymorphisms of-651C 〉 T in CD14 promoter contribute to the susceptibility to laryngeal cancer in Chinese population.