摘要
色氨酸(tryptophan,Trp)是一种特殊的氨基酸,它参与调节蛋白质的合成,同时在调节食欲与维持等方面都有很重要的作用。本文通过测定断奶仔猪的生长性能、血清中生化指标和游离氨基酸含量、肝脏色氨酸2,3-加双氧酶(tryptophan 2,3-dioxygenase,TDO)和肠道吲哚胺2,3-加双氧酶(indoleamine 2,3-dioxygenase,IDO)含量及Trp相关氨基酸转运载体基因表达,来探讨Trp对断奶仔猪氨基酸转运载体的影响。结果发现,添加0.2%Trp可显著增加日增重与日采食量,显著增加血液中色氨酸与亮氨酸的含量,显著降低缬氨酸与异亮氨酸的含量。0.1%Trp可显著增强肝脏TDO含量,0.2%Trp显著增加空肠IDO含量。添加0.2%Trp显著增加空肠基因ATB0,+、4F2hc、B0AT1和ASCT2表达(P<0.05)。综上,Trp能够促进相关氨基酸转运载体的表达,最终促进氨基酸的转运吸收。说明Trp可以通过上调氨基酸转运载体表达来调控机体健康。
Tryptophan（Trp） participates in the regulation of protein synthesis and plays very important roles in regulating appetite and maintaining health. The effect of Trp on amino acid transporters in weaned piglets was explored by determining growth performance, serum biochemical parameters, free amino acid content in the serum, tryptophan 2,3-dioxygenase（TDO） content in the liver, indoleamine 2,3-dioxygenase（IDO） content in the jejunum, and gene expression of amino acid transporters in weaned piglets. The results showed that addition of 0.2% Trp significantly increased the daily gain and daily feed intake, markedly elevated the levels of serum Trp and leucine, and dramatically decreased the levels of valine and isoleucine. The addition of 0.1% Trp and 0.2% Trp significantly increased TDO content in the liver and IDO content in the jejunum, respectively. The addition of 0.2% Trp markedly enhanced the gene expression of ATB0,＋, 4F2 hc, B0AT1, and ASCT2 in the jejunum（P〈0.05）. In conclusion, Trp can promote the expression of amino acid transporter-related genes and ultimately promote the transport and uptake of amino acids, indicating that Trp can regulate and control the health of the body by upregulating the expression of amino acid transporters.
出处
《现代食品科技》
EI
CAS
北大核心
2016年第2期40-45,共6页
Modern Food Science and Technology
基金
国家自然基金项目(31360552)
