RNA干扰肝细胞肝癌中PECAM-1的表达对肿瘤细胞侵袭及增殖的影响

Effects of PECAM-1 siRNA on the Invasion and Proliferation of Hepatocellular Carcinoma Cells

目的:探讨PECAM-1在肝细胞肝癌(Hepatocellular carcinoma,HCC)组织中的表达及意义。方法:选择2013年5月-2015年6月在我院接受治疗的HCC患者100例,收集肝癌患者HCC组织及癌旁组织,另选取100例正常肝脏组织作为对照组。应用免疫组织化学法检测PECAM-1在肝癌组织、癌旁组织以及正常肝脏组织中的阳性表达。利用小分子干扰RNA技术(si RNA)构建低表达的PECAM-1,并转染至肝癌细胞中抑制PECAM-1的表达。应用Transwell小室法检测肝癌细胞的侵袭能力,CCK-8法检测肝癌细胞的增殖能力。结果:PECAM-1在肝癌组织、癌旁组织及正常肝脏组织中呈不同程度阳性表达(P<0.05);PECAM-1在肝癌组织及癌旁组织中的表达显著高于正常肝脏组织,差异具有统计学意义(P<0.05);PECAM-1在肝癌组织中的表达显著高于癌旁组织,差异具有统计学意义(P<0.05);转染si RNA PECAM-1后,肝癌细胞中PECAM-1 m RNA的表达水平明显下降,PECAM-1蛋白表达也明显降低,差异具有统计学意义(P<0.05);转染si RNA PECAM-1后,肝癌细胞侵袭及增殖能力明显降低,差异具有统计学意义(P<0.001)。结论:PECAM-1在肝癌患者血清中高表达,PECAM-1 si RNA能够抑制肝癌细胞的侵袭及增殖能力,提示PECAM-1可作为预测肝癌发生及发展的临床指标。

Objective：To investigate the expression and significance of PECAM-1 in hepatocellular carcinoma（HCC）.Methods：100 patients with hepatocellular carcinoma who had taken the resection surgery in our hospital from May 2013 to June 2015 were selected,and the HCC cancer tissues and the para-carcinoma tissues were collected.Another 100 normal liver tissues were collected as the control group.Then the expressions of PECAM-1 in the tumor tissues,the para-carcinoma tissues and the normal liver tissues were detected by RT-PCR.The small interfering RNA（siRNA） was used to inhibit the expression of PECAM-1 in hepatocellular carcinoma.The invasion of hepatocellular carcinoma cells was detected by Transwell,and the proliferation of hepatocellular carcinoma cells was detected by CCK-8.Results：PECAM-1 positively expressed in HCC tissues,adjacent tissues and normal liver tissues（P0.05）.The expressions of PECAM-1 in HCC tissues and adjacent tissues were significantly higher than that of the normal liver tissues（P0.05）.The expression of PECAM-1 in HCC tissues was significantly higher than that of the adjacent tissues（P0.05）.The level of PECAM-1significantly decreased after transferred by PECAM-1 siRNA,and the invasion and proliferation of HCC cells significantly decreased,and the differences were statistically significant（P0.05）.Conclusions：PECAM-1 highly expressed in serum of patients with liver cancer,and siRNA PECAM-1 can inhibit the invasion and proliferation of HCC cells,that PECAM-1 can be used as a clinical indicator for predicting the occurrence and development of liver cancer.