环境丰富对SD大鼠生长和血液学指标的影响

Effect of Environmental Enrichment on Growth and Hematology in SD Rats

目的 探讨环境丰富长期作用对于SD大鼠生长、摄食、血常规及血生化指标的影响.方法 48只3周龄SPF级SD大鼠,随机分为2组,环境丰富组用磨牙棒和木丝筑巢材料进行环境丰富.试验期间测量体质量和摄食量,32周龄时解剖采腹主动脉血进行血常规和血生化检测,并测量主要脏器重量.结果 与对照组相比,环境丰富组雄性大鼠10～19周龄的摄食量显著增加,体质量增加但不显著,血清总蛋白、白蛋白和胆固醇显著升高.雌性大鼠在上述指标无显著差异.主要脏器方面,环境丰富组雌性心脏和肝脏重量显著高于对照组,雄性附睾重量显著高于对照组,其他脏器无显著差异.环境丰富组雌性大鼠红细胞、血红蛋白、血小板指标显著升高,而雄性大鼠在上述指标间则无显著差异.结论 环境丰富对SD大鼠生长和部分血液学指标有一点程度影响,实验设计时应慎重考虑环境丰富条件的选择.

Objective To study the effect of environmental enrichment on the growth and hematol, ogy of Sprague-Dawley （SD） rats. Method Forty-eight weaned 3-week old SD rats （male ： female = 1 ： 1） were allocated randomly to standard laboratory cages and enriched cages which were consisted of small sticks for grinding and wood-wool as nesting materials. The growth rate and food consumption were monitored. The rats were sacrificed at 32 weeks of age, and the indexes of organ weight, hematology and biochemistry were measured and analyzed. Results The food consumption of male rats in enriched cages was significantly increased between 10 and 19 weeks, but the weight gain was not significantly different compared to the standard group. The total serum protein, albumin and cholesterol were also increased significantly. No significant difference was found in the growth rate, food consumption or serum biochemistry in the female rats. The weights of epididymis of male rats and the weights of heart, liver of female rats were increased significantly. However, there was no significant difference for other organs. In addition, environmental enrichment showed significant impact on the erythrocyte, hemoglobin and platelet in the female rats, but not the male rats. Conclusions The environmental enrichment can alter the growth and hematology in SD rats to some degree, so the researchers need to pay attention on designing environmental enrichment strategies.