延边地区TP53基因-72密码子多态性与宫颈癌预后的相关性

The relationship between TP53-codon 72 polymorphism and prognosis of patients with cervical cancer

[目的]探讨延边地区TP53基因-72密码子多态性位点(TP53-rs1042522C>G)与宫颈癌预后的相关性.[方法]选择在延边大学附属医院和延边肿瘤医院接受治疗的宫颈癌患者403例(Ⅰ期21例、Ⅱ期245例、Ⅲ期132例、Ⅳ期3例、分期不详2例),给予抗癌治疗前采集外周静脉血,提取基因组DNA,利用聚合酶链反应-限制性片段长度多态性方法对TP53-rs1042522C>G进行分型.[结果]CC,CG和GG基因型在宫颈癌中的频率分别为23.3%,54.4%和22.3%,CC基因型总生存率显著低于GG,CG和CC+CG基因型(P=0.001,P=0.000 1).无病生存率分析结果显示,CC基因型总生存率显著低于GG,CG和CC+CG基因型(P=0.017,P=0.003),提示CC变异型宫颈癌患者总生存率和无病生存率显著低于其他基因型.[结论]TP53-rs1042522C>G可作为宫颈癌治疗后预测总生存率和无病生存率的生物标志物.

OBJECTIVE To investigate the relationship between TP53-codon 72 polymorphism （TP53-rs1042522 C〉G） and the prognosis of patients with cervical cancer in Yanbian area. METHODS The blood samples before treatment were collected from the 403 patients with cervical cancer （FIGO stage Ⅰ 21, stage Ⅱ 245, stage Ⅲ 132, stage Ⅳ 3, 2 cases not clear） who hospitalized in Affiliated Hospital of Yanbian University and Yanbian Cancer Hospital, and the extracted DNA was amplified by PCR followed by typing the RFLP of TP53-rs1042522 C〉G. RESULTS The ratio of CC, CG and GG genotype in cervical cancer was 23.3%, 54.4% and 22.3%, respectively. Overall survival （OS） rate of patients with CC genotype was significantly lower than that with GG, CG and CC ＋ CG genotype（P = 0. 001, P = 0.000 1）, and the disease free survival （DFS） rate of patients with CC CC＋ CG genotype （P = 0.017, P = 0. 003） genotype were dramatically lower than that could be a biomarker for prognosis of cervical genotype was significantly lower than that with GG, CG and The OS and DFS in patients with cervical cancer with CC with other genotype. CONCLUSION TP53-rs1042522 C〉 G cancer.